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13112 Basics of Suturing and Knotting in Ophthalmic Surgery
cd
Due to the limited surgical field that the
surgeon sees through the microscope, it is absolutely necessary to ensure a secure grip
the curve of the needle holder. This allows the needle to be guided precisely and securely dur-
ing tissue penetration. of the needle in the needle holder to achieve a safe guidance for gentle suturing with the lowest possible penetration force.
to clamp the needle at an angle (Fig. 12.1). In
these cases, the needle can only be securely
grasped for suturing with a straight needle
holder.

12.2.2 Needle Holder and Needle

area during clamping, it results in damage to the
Suturing begins with the selection of the nee­dle holder and the clamping of the needle. For corneal and conjunctival sutures, needle hold­ers without a lock have become established in ophthalmology. Many surgeons prefer curved needle holders. In this case, the needle must be grasped so that the convex curvature of the nee­dle holder and the needle tip point in the same direction and the needle forms a right angle with
ab
tip, making it blunt and hindering gentle tissue
penetration.
In case of improper handling, for example,
In special situations, it may be necessary
If the needle is accidentally grasped at the tip
after touching or bumping the needle tip, bead-like thickening can be observed under the operating microscope (Fig. 12.2). In this case, it is strongly advised to replace the damaged needle!
Fig. 12.1 The needle must form a right angle with the guiding line of the needle holder (a) to guide the needle pre- cisely and securely during tissue penetration. In special situations, it may be necessary to clamp the needle at an angle (bd). (Mod. after 2)
132 F. Wilhelm et al.
of the surgeon’s hand results in an arched move-
ment that follows the curvature of the needle,
allowing the tissue to be penetrated gently with
minimal pressure and without significant defor-
mation (Fig. 12.4).
In selected situations, it may be necessary to
suture “away from oneself.” For this, the needle
Fig. 12.2 Bead-like thickening results from damage to the needle tip
is held in the backhand position, and the arched
movement is performed through the pronation of
the hand. (This approach is necessary, for exam-
ple, when suturing corneal transplants.)
Grasping the needle too far at the proximal, round end leads to twisting and can cause bend­ing or even breaking during the suturing process. It is recommended to clamp it at the transition to the rear third (Fig. 12.3), but the optimal point
Care must be taken to pierce perpendicu-
lar to the wound edge to avoid shifting the wound surfaces and distorting the tissue
when tightening the knot. for grasping the needle also depends on the cur­vature, bending and breaking strength, and the consistency of the tissue to be sutured.
It has proven effective to first pierce the needle through only one edge of the wound and then release the needle so that the needle tip indicates
The firmer the consistency of the tissue to
be sutured, the closer to the middle the nee­dle must be clamped.
the direction in which the thread must be guided through the second wound lip to achieve an exact adaptation of the opposing fixation points (Fig. 12.5).
Then, the needle is grasped again behind the
12.2.3 Fundamentals of Microsurgical
Suturing
tip and precisely pierced through the second wound lip in the predetermined direction. This
approach is generally recommended for beginners During the suturing process, the penetration is dictated by the curvature of the needle. Since suturing is generally done “towards oneself,” the needle is held in the “forehand” position. It is distinguished whether the surgeon is sutur­ing with the left or right hand. The supination
and for experienced surgeons in difficult initial sit-
uations with swollen corneal stroma, as it results
in a secure and precise wound closure and mini-
mizes the extent of suture-induced astigmatism.
An exception is the double continuous diago-
nal suture in penetrating keratoplasty according
Fig. 12.3 It is recommended to grasp the needle between the middle and rear third of the curve
13312 Basics of Suturing and Knotting in Ophthalmic Surgery
a
b
Fig. 12.4 The suturing movement follows the needle curvature: (a) vertical insertion of the needle tip, (b) gentle penetration of the tissue through the arched supination movement of the surgeon with the needle
running superficially and the other deep, results
in a clean adaptation without gaping.
For individual sutures on the cornea, it is important that the wound edges are adapted at the same depth with the opposite side (Fig. 12.6). The extent of the inner wound gap depends on the depth of the suture guidance.
For this purpose, the surgeon can enlarge the image under the operating microscope as needed so that the boundary between the stroma and the Descemet’s membrane is clearly visible in depth. The needle can then be controlled to pierce in and out closely in front of it, achieving a secure
Fig. 12.5 The needle is pierced through the first wound lip and then released by the needle holder to indicate the direction in which the suture must be guided through the second wound lip, ensuring that there is no shifting of the wound edges against each other after the knot is tied
wound closure over the entire corneal thickness.
Since gaping wounds on the eye sur-
face are filled with granulation tissue
and often colonized by bacteria, incom­to Hoffmann [7]. In this case, the needle is pierced in and out at an angle to the wound edge. The guidance of the two sutures, one
plete adaptation results in fistulas and
step formations, which can cause a for-
eign body sensation with subsequent
134 F. Wilhelm et al.
ab c
Fig. 12.6 Exact adaptation of opposing wound edges deep lamellar before the DM (a) ensures a tight wound closure, which is not the case with different stitch distances (b) or penetration depths (c). (Mod. after 9)
vascularization in the patient and induce higher astigmatism!
12.2.4 The limbus suture is started
rst!
Before closing any wound, it must be clarified at which point the suturing will begin. In the case of a cornea-sclera wound due to an injury, the first suture must always be the limbus suture [12]. After that, it can be decided how many sutures will likely be needed. These should be distrib­uted successively according to the wound course to arrange the distances between the individual sutures in such a way that no tissue overlap results [4]. Distortions and folds of the wound edges, which mainly affect the cosmetic result on the skin, cause significant astigmatism on the cornea.
Beginners in ophthalmic surgery should defi­nitely opt for single button sutures again: inter­rupted on the cornea, the conjunctiva and on the skin.
It is important to arrange the stitches in such
a way that a secure wound closure is achieved with as few sutures as possible (Fig. 12.7).
With increasing experience, the single button
sutures can then be replaced by continuous
Fig. 12.7 When adapted by EKN, the wound edges are sealed only in the area compressed by the suture. Leaks can result in between! (Mod. after 4)
13512 Basics of Suturing and Knotting in Ophthalmic Surgery
or cross sutures. In the case of swollen and
jagged wound edges, it may be necessary
to temporarily place individual sutures as
“assembly aids” or situational sutures to better
place the final adaptation threads afterward.
Subsequently, the situational sutures should be
removed again. Especially when placing sev-
eral single button sutures with the same direc-
tion of pull, loosening of previously placed
threads can occur after the corneal stroma has
de-swollen. In this case, it must be decided
which suture needs to be replaced.

12.3 Knots

When tying a knot, some basic rules must be observed. The thread ends must be sufficiently long to form the knot at all. The length of the longer thread end required to execute the knot and grasp the short end depends on the number of planned turns (Fig. 12.8).
The second thread end should, if possible, not be too long, as loops can form when pulling through, which can tighten and may not be easily loosened. The short thread end is grasped with the needle holder, then the long end is wound around the instrument, and the short thread end is pulled through the loop resulting from the winding [6]. To practice the movement sequence for the instrument knot, dry exercises on suitable models are recommended (Fig. 12.9).
The needle itself must not be used to tie the
knot! It should be placed within the micro­scope field so that it does not need to be searched for after cutting.
When tying with instruments, special care
must be taken to ensure that the edges of the needle holder and forceps are smooth, as otherwise the vulnerable thread can eas­ily be damaged. This is especially important
Fig. 12.8 When tying with instruments, the thread ends must be sufficiently long to form the knot at all!
136 F. Wilhelm et al.
ab c

12.3.1 The First Knot

The first knot should be placed exactly as a surgical knot in a double winding to increase the friction between the thread loops and thus counteract the separation of the wound edges due to tissue tension. This knot must always be tightened in the “running direction” of the loop. Then it must be checked whether the tension is sufficient or whether the wound edges distance themselves from each other again due to tissue tension.
Fig. 12.9 The movement sequence for the instrument knot can be practiced on the model in the dry lab
when very thin monofilament suture mate­rial is used. Monofilament threads should never be stretched too much, as they can twist and possibly break.
Depending on the direction in which the thread is looped during the execution of the second winding, a “granny knot” or a “reef knot” is formed [1]. While the reef knot usually adapts the wound edges very stably through two indi­vidual loops, the granny knot leaves the possibil­ity open that it can be tightened even more after forming the second throw (Fig. 12.10). The reef knot is also known from sailing as a square knot [5], in which the two overlapping thread wind­ings lock each other.
If the wound edges gape apart, one end of
the thread can be passed under the opposing
thread again to achieve an additional wind-
ing. This is gentler than reopening and re-
looping the knot.
At this point, the question arises as to how many windings are necessary for the knot at all.

12.3.2 Number of Windings

The number of windings depends on both the type of suture material and the tension of the tis­sue to be sutured. With high tissue tension and monofilament material, more windings are nec­essary for a tight wound adaptation.
In principle, a knot should be tied with the smallest possible number of windings to make
Fig. 12.10 a,b a Granny knot. b Square knot. c Surgical knot. (Mod. after 1)
13712 Basics of Suturing and Knotting in Ophthalmic Surgery
it overall smaller. This is not significant for skin sutures that are removed early. However, large knots on the conjunctiva and cornea lead to pain and blepharospasm due to the foreign body irri­tation and are often not tolerated by patients.

12.3.3 Smooth or Overhand Knot

When forming the knot, a distinction is made between a smooth knot and an overhand knot in the first winding (Fig. 12.11). If the threads are not tightened in the running direction, the knot overhands and slides on the second thread end (Fig. 12.12).
In principle, the two consecutive loops of
a knot should differ by only one winding (Fig. 12.10), otherwise it overhands when tightened and does not hold. However, the knot becomes bulkier due to the increased use of suture material, which is why it is also recommended to deviate from this rule for corneal sutures if necessary [8].

12.3.4 Burying the Knot

Corneal threads should be buried. Therefore, it is necessary to design the knot particularly care­fully here, to tighten it securely, and to cut the
thread ends as short as possible. This is best achieved by cutting the tightly held monofila­ment thread close to the knot. It is broken over the edge of a microscalpel (Fig. 12.13). In doing so, the surgeon can precisely determine the dis­tance to the knot, whereas when using scissors, the knot may be obscured by the branches of this instrument, making it difficult to accurately estimate the length of the thread ends at the knot.
This approach is recommended exclusively
for monofilament sutures to shorten the ends with a single, controlled action.
When using multifilament suture material on the skin and conjunctiva, this makes little sense, as the thread would otherwise split into its indi­vidual fibers. Here, a suitable pair of scissors should be preferred (Fig. 12.14).
When sinking the knots into the corneal tis­sue, care should be taken to ensure that the threads can be removed as gently as possible later. Therefore, after the knot has been rotated into the deeper stroma, it is then rotated back up to just below the surface. This aligns the thread ends in the direction of pull, whereas if this retraction is omitted, they act like small barbs that, after fibrosis has formed around the knot, make it difficult to remove the threads at a later time (Fig. 12.15).
138 F. Wilhelm et al.
a
b
c
d
Fig. 12.11 (ad) left side smooth/square knot, right side overhand knot. (Mod. after 4)
13912 Basics of Suturing and Knotting in Ophthalmic Surgery
c
b
a
Fig. 12.12 a–c With monofilament suture material, an additional securing loop c follows the adaptation knot a and the securing loop b. (Mod. after 4)
Fig. 12.13 To break the monofilament suture close to the knot over the edge of the microscalpel, the thread must be held tightly!
140 F. Wilhelm et al.
cd
Fig. 12.14 The shortening of the thread ends to the appropriate length is done with scissors for skin and con­junctival sutures
ab
Fig. 12.15 a–c After tying, the knot is tightened a and rotated into the corneal stroma b. This aligns the thread ends so that they act like barbs c, which, particularly due to fibrosis after remodeling processes during wound healing, makes it difficult to remove the knots. Therefore, the knots are rotated back up to just below the corneal surface by pulling the thread in the opposite direction, aligning the ends in such a way (d) that the thread along with the knot can be more easily removed

References and Further Reading

1. Becker T (1989) Kurzgefasster Operationskurs. Johann Ambrosius Barth Leipzig. 315 S
2. Castroviejo R (1968) Keratoplastik. Thieme, Stuttgart, p 453 S
3. Domarus v D et al (1996) Trauma, Operationen und Wundheilung des Auges. In: Naumann GOH (Eds) Pathologie des Auges, Band I, pp 301–377. Springer, Berlin
4. Eisner G (1978) Augenchirurgie. Einführung in die operative Technik. Springer, Berlin
5. Friedrichsen W (1986) Das Segelhandbuch. Hoffmann und Campe, Hamburg