Roman S. Kozlov
Eugeny
A. Fedosov
General
MICROBIOLOGY&
IMMUNOLOGY
Guidelines for students
2004
УДК
579
О
28
Рецензенты:
д.
м. н., профессор кафедры микробиологии
Российской
медицинской академии
последипломного
образования С.
В.
Сидоренко;
д.
м. н.,
профессор,
заведующий
кафедрой
микробиологии,
вирусологии
и иммунологии
Санкт-Петербургского
государственного
медицинского
университета
им.
акад.
И. П. Павлова
В.
В.
Teц
O
28 General
microbiology and immunology: guidelines for students. -
Смоленск:
Смоленская государственная медицинская
академия,
2004
г.
- 160 c.
Рекомендовано
Учебно-методическим объединением по
медицинскому
и фармацевтическому
образованию Вузов России
в
качестве учебного
пособия для
иностранных студентов медицинских
вузов.
УДК
579
©
2004 Смоленская
государственная
ISBN
5-86064-085-4 медицинская академия
CONTENT
Introduction 4
Practical session No. 1 5
Practical
session No. 2 13
Practical
session No. 3 22
Practical
session No. 4 26
Practical
session No. 5 37
Practical
session No. 6 49
Practical
session No. 7 60
Practical
session No. 8 76
Practical session No. 10 83
Practical session No. 11 95
Practical session No. 13 116
Practical
session No. 14 139
Practical
session No. 15 146
Practical
session No. 16 152
3
These guidelines have been specially developed in order to assist
students,
tutors and everyone who is interested in the subject
in better understanding of
general microbiology and immunology.
Each of the practical sessions has been designed in a same way. It
includes a
description of plan, main objectives, educational
tasks, demonstrations, students’
activities and control
questions. Foreword notes and methodical guidelines take
a
central place in each topic and, not being a substitute for the
textbooks, make
it more convenient to prepare for practical
session and self-study.
Authors will be very grateful for all comments, suggestions and
critical
remarks, which will allow us to improve these
guidelines. All of these should
be addressed to authors:
Department of Microbiology, Smolensk State
Medical Academy,
214019, Russian Federation, Smolensk, 28 Krupskaya
Street,
Email: roman_s_kozlov@hotmail.
com
4Introduction
PRACTICAL
SESSION No. 1
Subject
and objectives of microbiology. Rules of work in the
microbiological
laboratory. Forms of bacteria. Simple staining
techniques.
Plan
of the session
Rules of organization and equipment of microbiological laboratory.
Rules of work in the microbiological laboratory.
Modern methods of microscopic examinations.
Main forms of bacteria.
Preparation of bacterial smears and simple staining techniques.
Foreword
notes
Medical
microbiology has the following main objectives:
Study of biological properties of microorganisms and processes of
their
interactions with human organism and the environment.
Microbiological diagnosis of infectious diseases.
Development of methods of detection of microorganisms in
the
environment.
Development of methods of specific prophylaxis and treatment
of
infectious diseases.
Organization of production of bacterial preparations, antibiotics
used
for diagnostic, therapeutic and prophylactic purposes.
Students, who visit Department of Microbiology, must follow rules
accepted
in microbiological laboratories where the potentially
infectious materials are present.
One of the stages of microbiological diagnosis of infectious
diseases is the
study of morphology and staining
characteristics of microorganisms. To achieve
the above,
microscopic methods are used.
Main
objective of the session
To make students accustomed with principles of organization,
equipment
of microbiological laboratory and rules of work in
it, main microscopic methods,
preparation of bacterial smears,
simple staining techniques and main
morphological forms of
bacteria.
Educational
tasks
To know: 1.
Principles of organization and purpose of microbiological
laboratory.
Rules of work in the microbiological laboratory.
Types of modern microscopes. Principles of functioning
of
electronic, fluorescent, phase-contrast, dark-field
microscopy.
Main characteristics of light microscope.
5