4. Materials for auditory self-work

4.1. List of study practical tasks necessary to perform at the practical class.

It’s necessary for work: microscope, aggregatograms, Goryaev’s chamber, covering glasses, plasma rich in platelets, aggregatograph.

Task 1. Bleeding duration determining (by Duke).

To prick the finger on the depth of scarificator point. To absorb flowing blood in every 30 sec in filter paper. Under norma bleeding duration is 2-4 minutes.

Task 2. Aggregatogram analysis principle.

Diagnostic value: it can give information about thrombocytes qualitative defects and platelets dysfunctions, platelets quantitative changes at different pathologic processes. This platelets’ function disturbance will be accompanied by hemorrhages from gums, oral mucosa.

Main aggregatogram indexes:

1. Aggregation angle (α – L on the figure) – index which reflects aggregation coming velocity; it is determined by aggregatogram curve ascent steepness after aggregation inductor addition.

2. Aggregation time (t₁) – it is measured from aggregation initiating (beginning) till point on aggregatogram curve corresponding to maximal aggregation; it characterizes aggregation degree.

3. Aggregation latent period duration (t) – index which reflects processes not-registrated on photoelectrocalorymeter (PEC).

4. Desaggregation angle (в) – index which reflects desaggregation process velocity.

5. Aggregation height (h) – index reflecting aggregation degree; it corresponds to thrombocytic plasma optical density decreasing descent in course of aggregation.

1. 

FIGURE 12. Aggregatogram.

5. Literature recommended:

1. Lecture course.

2. Mistchenko V.P., Tkachenko E.V. Methodical instructions for dental students (short lecture course).-Poltava, 2005.-P.41-42.

3. Mistchenko V.P., Tkachenko E.V. Methodical instructions for medical students (short lecture course).-Poltava, 2005.-P. 71-73.

4. Mistchenko V.P., Tkachenko E.V. Blood system Physiology //Methodical recommendations to practical classes for students of medical and dental departments.-Poltava, 2005.-20p.

5. Kapit W., Macey R.I., Meisami E. The Physiology Colouring Book: Harpers Collins Publishers, 1987.-P. 138.

6.Guyton – Ganong – Chatterjee. Concise Physiology /Ed. By Dr Raja Shahzad Gull: M.B.B.S., F.C.P.S., King Edward Medical College.-Lahore, 1998 (1^st Edition).-P.197-198.

7. Stuart Ira Fox. Human Physiology.-8^th Ed.-McGrawHill, 2004.-P.369-370, 374.

8. Seeley R.R., Stephens T.D., Tate P. Essentials of Anatomy and Physiology.-The 3^rd Ed.-McGraw Hill, 1999.-P.293-294, 300.

6. Materials for self-control:

Control questions

1. Thrombocytes, amount, structure.

2. Platelets functions.

3. Vascular-platelet hemostasis mechanism.

4. Microcirculative hemostasis and its significance in dentistry.

5. Thrombocytopoiesis and its regulation.