- •Content
- •Сontent module 11: blood system physiology
- •Lesson 31
- •Blood physical-chemical features investigation
- •2. Study aims:
- •3.1.Basic knowledge, skills, experiences, necessary for study the topic:
- •3.2.Topic content
- •Introduction
- •Variations in plasma protein level
- •Increase in all fractions
- •Materials for auditory self-work.
- •Task 1. To get acquainted with blood taking technology for analysis performance.
- •Task 2. To determine erythrocytes osmotic resistance.
- •Task 3. Velocity sedimentation rate (vsr) determining.
- •2. Literature recommended:
- •Materials for self-control:
- •Lesson 32
- •Erythrocytes number and hemoglobin concentration investigation
- •Introduction and normal value
- •Variations in number of red blood cells
- •Variations in size of red blood cells
- •Variations in shape of red blood cells
- •In postnatal life and in adults
- •2. Hormones:
- •1. Vitamin b12 (Cyanocobalamin)
- •2. Intrinsic Factor of Castle
- •3. Folic Acid
- •Neural-humoral erythropoiesis regulation
- •Erythropoiesis inhibitors
- •Iron metabolism
- •Task 1. To determine erythrocytes amount in blood.
- •Task 2. Hemoglobin content determining in blood.
- •Task 3. To estimate blood color index.
- •Lesson 33
- •Blood groups belonging investigation
- •2. Study aims:
- •Table 2. The blood groups with their genotypes and their constituent agglutinogens and agglutinins
- •Materials for auditory self-work
- •4.1. List of study practical tasks necessary to perform at the practical class.
- •Task 2. To determine rhesus-factor while express-method usage.
- •Task 3. To perform probe on individual compatibility.
- •Literature recommended:
- •Materials for self-control:
- •Lesson 34
- •Leucocytes number, leucocytic formule investigation
- •2. Study aims:
- •Variations in the count of white blood cells
- •Innate immunity
- •Introduction
- •Immunization
- •1. Interleukins
- •2. Interferons
- •Acquired immunodeficiency syndrome (aids)
- •Differentiated leucocytes ageing changing in children
- •Leucocytes functions significance in dentistry
- •Materials for auditory self-work
- •Task 1 Leucocytes estimation in Goryaev’s chamber
- •5. Literature recommended:
- •Lesson 35
- •Platelets and vascular-platelet hemostasis investigation
- •1. The topic studied actuality.
- •Complications after teeth extraction in patients with microcirculative hemostasis disorders
- •2. Study aims:
- •Error: Reference source not found
- •4 Forms of platelets:
- •Hemostasis
- •Platelet plug formation
- •Vascular-platelet hemostasis
- •Vessels temporary spasm:
- •Vessels injury
- •Adhesion
- •Platelets
- •Releasing reaction
- •4. Materials for auditory self-work
- •4.1. List of study practical tasks necessary to perform at the practical class.
- •Task 1. Bleeding duration determining (by Duke).
- •Task 2. Aggregatogram analysis principle.
- •5. Literature recommended:
- •6. Materials for self-control:
- •Lesson 36
- •Blood coagulation investigation
- •Physiological bases of measurements at prolonged bleeding after tooth extraction
- •Physiological basement of patients preparation to tooth extraction at blood diseases
- •Complications occurring after tooth extraction in patients with blood coagulation disorders
- •2. Study aims:
- •3.1.Basic knowledge, skills, experiences, necessary for study the topic:
- •Topic content
- •Plasma blood coagulation factors
- •Materials for auditory self-work
- •Task 1. To study thromboelastogram.
- •5. Literature recommended:
- •6. Materials for self-control:
- •Lesson 37
- •Differentiated coagulogram. Disseminated intravascular coagulation (dic) syndrome
- •2. Study aims:
- •3.1.Basic knowledge, skills, experiences, necessary for study the topic:
- •Topic content
- •Main pathological processes and influences accompanied by dic-syndrome development (dic ethiology)
- •Dic types:
- •4. Materials for auditory self-work
- •4.1. List of study practical tasks necessary to perform at the practical class.
- •Task 1. Coagulogram for dic-syndrome (disseminated intravascular coagulation) diagnostics
- •Task 2. To assess hematomic hemorrhagia type.
- •Task 3. To assess microcirculative (petekchio-spotted) haemorrhagia type
- •Task 4. To assess mixed (microcirculative-haematomic) bleeding type
- •Task 5. To get acquainted to doctor tactics at vasculite-purpure and microangiomatose bleedings types
- •5. Literature recommended:
- •6. Materials for self-control:
- •Lesson 38
- •Fibrinolysis and anticoagulants. Blood coagulation and fibrinolysis regulation
- •2. Study aims:
- •3.1.Basic knowledge, skills, experiences, necessary for study the topic:
- •3.2. Topic content
- •Table 5. Main primary physiological anticoagulants
- •Plasminogen
- •Hageman-dependent
- •Hageman-independent
- •Plasmin
- •Task 1. Blood fibrinolytic activity determining.
- •Task 2. Fibrinolytic bleeding laboratory diagnostics principles.
- •Task 3. Getting acquaintance with some tests characterizing hemostasis anticoagulant link
- •5. Literature recommended:
- •6. Materials for self-control:
- •Lesson 39
- •Total blood
- •2. Study aims:
- •3.1.Basic knowledge, skills, experiences, necessary for study the topic:
- •3.2. Topic content
- •Coagulogram changes in children
- •In mature new-borned
- •In immature new-borned:
- •Total blood
- •4. Literature recommended:
- •Lesson 40
- •Practical skills on blood system physiology
- •Glossary
- •Blood system physiology
- •Tests on blood physiology
Materials for auditory self-work
4.1. List of study practical tasks necessary to perform at the practical class.
Materials and methods: blood, china plate, scarificators, standard group specific sera, subject glasses, glass sticks, antirhesus serum, Tsoliclones anti-A and anti-B.
Investigative object: human being.
Task 1. To determine human being blood group on ABO system:
A. By means of group-specific sera:
To pour blood sera on china plate correspondingly to blood groups designations. To process the finger and to prick it with scarificator. To place blood drop in a plate central nest. To add blood to the serum (in a correlation of 1:10) with clean subject glass separate angles. To get the plate rocking in course of 3-5 minutes. To mark the nets where agglutination reaction occurred. To determine blood group.
B. By means of Tsoliclones anti-A and anti-B:
To pour Tsoliclones anti-A and anti-B on 1 big drop (0,1 ml) on the plate under corresponding writings. To pour investigated blood near drops in 10 times less than antibodies drop. To mix with glass stick (different in every drop). To get the plate rocking in course of 2-3 minutes. The result in every drop may be positive or negative. To determine blood group.
Results interpretation:
if agglutination reaction is absent with all group-specific sera and with all (both) Tsoliclones, than given blood group doesn’t contain antigens A and B, thus it belongs to blood group 0(I);
if agglutination reaction took place with I and III serum and Tsoliclone anti-A, then given group contains antigen A and belongs to A(II) group;
if agglutination reaction occurred with I and II sera and with Tsoliclone anti-B, then given blood group belongs to B(III) blood group;
if agglutination reaction took place with sera of I, II, III groups and with both Tsoliclones, then investigated blood contains both antigens A and B and blood belongs to the group AB (IV).
Task 2. To determine rhesus-factor while express-method usage.
To pour 1 drop (20 divisions of Panchenkov’s capillary pipette) of anti-rhesus serum to the investigated blood on test tube floor. To shake up the test tube and to turn over several times so that its content was flowing on the walls. To add 2-3 ml of 0,85% solution of NaCl solution in 3 min. To mix test tube content after its 1-2-folded turning over. Don’t shake up!
To perform results assessment on agglutination absence or presence (large flakes on the background of enlighten liquid).
Task 3. To perform probe on individual compatibility.
To pour recipient blood plasma on subject glass. To add donor blood drop less in 10 times than plasma (in a correlation of 1:10) to this plasma. To evaluate their compatibility.