- •High-Purity Separation of Phospholipids by Preparative High-Performance Liquid Chromatography
- •Introduction fusiogenic properties of the sperm [5].
- •Table 1. Linear Gradient Conditions with Various Analytical and Preparative Packings
- •Column Efficiency and Resolution
- •In order to investigate the resolutions of pe, pi, and pc with the packing materials, the following equation was used,
- •Conclusion
- •Acknowledgements
Conclusion
The optimum experimental conditions of the mobile phase compositions and gradient conditions were investigated to separate phospholipid from lecithin with the different particle sizes. The ternary mobile phase of hexane, methanol, and IPA were used as mobile phases. The optimum composition of mobile phase for the separation of phospholipids from lecithin was determined on the basis of resolutions and separation times. The better resolutions were obtained when the particle size of packing material is smaller and more uniform. Among the three preparative packings, 15 n m packings is recommended for the preparative work. The experimental result of the operating condition might be extended to commercial scale. As the higher pressure drop was accompanied by larger packings, a pump with high- pressure rating should be necessary.
Acknowledgements
This work was performed in High-Purity Separation Lab., Inha University, Inchon, Korea. Also this work was financially supported by Korea Research Foundation Grant (KRF-2000-041-E00338).
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