Bank of Krok testing to module n1

Morphology of bacteria

7. Immunofluorescence reaction is widely used for express-diagnostics of many bacterial and viral infections. Choose the type of the microscope needed for this reaction.

A.Light microscope.

D.Phase-contrast microscope.

B. Electron microscope.

C. Fluorescence microscope.

E. Dark-field microscope.

10. The essential drawback of the microscopy method of infection diagnostics is insufficient informativeness caused by morphologic similarity of many mi­croorganisms. Which of the following can increase the informativeness?

A. Radioimmunoassay.

B. Coombs' test.

C. ELISA.

D.Opsonization reaction.

E. Immunofluorescence testing.

*Immunofluorescence test belongs to widely used rapid (express) methods of infectious diseases diagnostics. Which properties of bacteria are estimated for their identification at this type of testing?

Morphological and tinctorial

Morphological and antigenic

Antigenic and immunogenic

Cultural and biochemical

*During microbiology lesson students have acquainted with microscopy method of laboratory diagnostics. Which property of bacteria may be studied with this method?

Morphological, tinctorial

Cultural

Antigenic

Toxigenic

Biochemical

20. Furuncle pus smear showed spherical microbes arranged in grape-like clusters. Name these microorganisms.

A.Staphilococci.

B. Diplococci.

C. Micrococci.

D.Streptococci.

E. Tetracocci.

*At microscopy of the wound discharge gram-positive spherical cells arranged in “grape-like clusters “ have been revealed. Which group can seen microorganisms be belong to?

Staphylococcus

Clostridia

Streptococcus

Mycoplasma

Gonococcus

70. Students have been proposed to stain a mixture of bacteria by Gram's method and explain the mechanism of staining at a microbiology practical class. Name the morphological structures of bacteria that cause Gram-negative or Gram-positive staining of bacteria.

A.CPM (cytoplasm membrane).

B. Cell wall.

C. Capsule.

D. .Flagella.

E. Cytoplasm.

*At laboratory diagnostics of many infectious diseases Gram staining technique is used. Which structure of microbial cell is responsible for ability of taking a dye?

Cytoplasmic membrane

Cell wall

Mesosomes

Capsule

Plasmids

*04* 2013* At staining of the smear from sputum collected from the patient with suspicious lobar pneumonia such dyes and reagents have been used as crystal violet and Lugol’s iodine solutions, 96⁰ alcohol, and fuchsine solution. Which method of staining has been used in this case?

Loeffler’s

Neisser’s

*Gram’s

Ziehl-Neelsen’s

Romanovsky-Giemsa’s

* Microorganisms surrounded with capsule have been revealed in the smear prepared from the sputum of the patient with croupous pneumonia. Which chemical compound of revealed cellular organella is?

RNA

Polysaccharides

Peptidoglycane

Lipids

DNA

*2013* *Microscopic investigation of pathological samples stained with Burri-Hins´ technique from the patient with suspicious plague is made into the laboratory of life-threatining diseases. Which property of causative agent does this method allow to study?

Capsule formation

Spore formation

Acid-fast property (acid resistance)

Alkaline resistance

Volutine granules presence

*b-12 At microscopy of bacterial culture fusiform spore-forming blue- violet after staining by Gram microorganisms have been revealed. Which kind of microorganisms has been revealed?

Streptococci

Spirochetes

Clostridia

Actinomyces

Diplococci

*b-12 Rod-shaped blue microorganisms with terminal spherical red components are seen in the smear stained by Aujesky´s method. Which name of these components is ?

Spores

Flagella

Pili (fimbria)

Capsules

Mesosomes

*08* A specimen stained by Ozheshko (Aujesky) method contains rod-like microorganisms stained blue with round terminal components stained red. What are these components called?

Cilia

Capsules

Flagella

Spores

Mesosomes

*At staining of the sputum smear the such dyes and reagents have been used as: Ziehl´s fuchsine and methylene blue solutions, 5% sulpharic acid solution. Which method of staining has been used?

Ziehl-Neelsen´s

Hins-Burri´s

Gram´s

Peshkov´s

Neisser´s

*The pure culture of Corynebacterium diphteria has been isolated from the patient. After smear preparation and staining by Neisser´s the next inclusion bodies have been revealed:

Starch

Glycogen

Sulfur

Volutin (polyphosphates)

The drops of neutral lipids

Physiology

Bacteriological method (pure culture isolation) is used for laboratory diagnostics of many infectious diseases. Which purpose of the first stage of investigation?

Seeding of the collected samples

Obtaining of the separated colonies

Microscopy of the investigated samples

Isolation and accumulation of the pure culture

Identification of the isolated culture

*DNA-polymerase from Thermus aquaticus is important reagent of the polymerase chain reaction (PCR). This microorganism is capable of growing at 100 ⁰C and higher and it is:

Thermophilic

Mesophilic

Psychrophilic

Halophilic

Chemolytotrophs

Genetics

The event, at which prophage may replicate with bacterial chromosome in the same time, is known as:

Transfection

Lysogeny

Transformation

Modification

Transduction

*It is known bacterial cells include additional genetic structures, which either exists as independent element autonomically from chromosome or integrates with it, and supplements such cell additional property. What is the name of this additional elements?

Prophages

Nucleotides

Is-sequences

Plasmids

Migrating genes

*The procedure of human gene incorporation into bacterial genome with bacteriophage is used in biotechnological processes for production of therapeutic and prophylactic medicines. What is the name of such process?

Transduction

Transformation

Translation

Conjugation

Deletion