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Mandatory Activities.doc
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Seed Germination

Seed germination is the restart of growth by the plant embryo using the food stored in the seed.

Water, oxygen and a suitable temperature are the major factors for successful germination.

Water

  • Plant cells are 90% water.

  • Water is essential for increase in cell number during growth.

  • Water is needed for food reserve digestion and transport of nutrients to the growing points.

Oxygen

  • Oxygen is needed for efficient ATP production from the reserve food.

  • Fermentation is nineteen times less efficient than aerobic respiration.

Suitable Temperature

  • Required for optimum enzyme activity and so for optimum growth.

  • Some of the enzymes are involved in the digestion of the complex food reserve.

  • Other enzymes are involved in growth and ‘housekeeping’ activities.

Stages of Seedling Growth

Textbook Diagram: seed germination

  • Conditions for germination are suitable and dormancy is over.

  • Water and oxygen enter the seed.

  • Metabolism speeds up.

  • Enzymes digest the complex food reserve (starch, protein, lipid).

  • The digested food is transported to the growing areas of the embryo.

  • The food is used for growth.

  • Most of the food is used in aerobic respiration resulting in loss of dry weight.

  • The radicle grows out forming a rooting system.

  • The plumule emerges forming a shoot system.

  • Photosynthesis begins.

  • Dry weight increases and seedling is independent when photosynthesis is greater than respiration.

Mandatory Activities

To Investigate the Effect of Water, Oxygen and Temperature on Seed Germination

Textbook Diagram: set up of the apparatus

  • Four small clear glass jars kept in darkness.

  • Jar A: water-soaked seeds on soaked cotton wool, open to air at 20ºC.

  • Jar B: dry seeds on dry cotton wool, open to the air at 20ºC.

  • Jar C: 4°C – water-soaked seeds on soaked cotton wool (in a fridge) open to the air.

  • Jar D: water-soaked seeds at 20°C in a sealed jar that does not have oxygen in the air – oxygen removed by pyrogallol or wet iron filings on filter paper.

  • One week later check the results.

Results

  • A: water, oxygen and suitable temperature together present / germination.

  • B: no water / no germination.

  • C: low temperature / no germination.

  • D: no oxygen / no germination.

Conclusion: water, suitable temperature and oxygen are together required for seed germination.

To Show Digestive Activity During Seed Germination

Textbook Diagram: set up of apparatus

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