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  • Bias: purposely choosing sample sites to get ‘good results’ or avoid work.

  • Too Few Sample Sites: may not give accurate representative results.

  • Surveyor Variation: students vary in ability, commitment and interest.

  • Equipment Quality: measurement and trapping success will be affected.

  • Changing Nature: results may depend on the time of day, season or year.

  • Chance: cannot survey every square centimetre so even with many sites some species may be missed.

  • Improper Trapping Techniques: all evasive species may not be captured and/or insufficient numbers captured in follow up surveying.

The Cell

Cell Theory

All living organisms are made up of cells and new cells are produced when live cells divide.

Textbook Diagrams: animal and plant cell as seen with light and electron microscopes.

Light Microscope Study Components of Animal Cells: cell membrane, cytoplasm, nucleus, chromosomes.

Extra Components of Plant Cells: cell wall, chloroplast, vacuole.

Electron Microscope Study Extra components and details:

Cell membrane, mitochondrion, nuclear pores, ribosomes, chromatin, details of chloroplast mitochondrion structure, double membrane nature of nuclear envelope and outline structure of cell membranes.

(The term protoplasm is useful and refers to the cell membrane, cytoplasm and its contents and the nucleus. The large vacuole of plant cells is not included.)

Cell Structure

Cell Membrane

  • Semipermeable: water, oxygen and carbon dioxide freely pass through it, many other chemicals cannot.

  • Selectively permeable: controls entry and exit of specific materials.

  • Keeps cell contents together allowing efficient coordination of its activity.

  • Maintains the interior of the cell at a suitable constant composition for efficient metabolism

Cytoplasm

  • The living contents of a cell excluding the nucleus and large vacuoles.

  • A complex solution in which the cell’s organelles are suspended.

  • Many biochemical processes take place here, e.g., glycolysis and fermentation.

  • About 90% water.

Nucleus

  • Contains DNA, the hereditary material.

  • The genes are present in the DNA.

  • Controls the cell’s structure and metabolism.

DNA (deoxyribonucleic acid) is visible as chromatin in active cells and as chromosomes during mitosis and meiosis.

A nucleus is not present in a red blood cell or phloem sieve element.

Nuclear Pores Large molecules can pass between the cytoplasm and the nucleus through these pores. Examples: RNA from nucleus to cytoplasm and nucleotides from cytoplasm to nucleus.

Chromatin Chromatin is the very fine thread-like combinations of DNA and protein in non-dividing nuclei. The protein assists in the efficient packaging and regulation of DNA activity. A human nucleus contains 46 such fine threads of chromatin.

Chromosomes A chromosome is a ‘condensed chromatin’ thread visible during mitosis and meiosis.

Haploid nuclei have one set of chromosomes i.e. one of each kind of chromosome. Each chromosome has a unique set of genes. Each gene has a specific locus – it is on a particular chromosome at a specific site.

Diploid nuclei have two sets of chromosomes i.e. two of each chromosome.

Symbol for haploid: n Symbol for diploid: 2n

The nuclei of human somatic cells are diploid (2n). Each human nucleus has 46 chromosomes i.e. two sets of 23 chromosomes. One set was received from the mother in the haploid egg cell, the other in the father’s haploid sperm cell.

Sex Chromosomes: the 23 rd pair. Female: XX Male XY

Mitochondria:

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