
- •1.The basic properties of microorganisms. Factors ubiquitous of microorganisms
- •3.Major fields of theoretical and applied Microbiology
- •4.Major Characteristics of Eukaryotes and Prokaryotes
- •6.Sphere -haped bacteria. The variety of forms, their arrangement, examples, a brief description
- •7.Curved-haped bacteria. The variety of forms, their arrangement, examples, a brief description.
- •8.Plazma (cytoplasmic) membrane. Structure. Functions. Destruction of the plasma membrane by antimicrobial agents
- •9.Movement of materials across membranes. Simple diffusion. Facilitated diffusion.Osmosis.
- •10)Movement of materials across membranes. Active transport. Group translocation.
- •12.The Golden age of microbiology. The discoveries of Pasteur and Koch. Their significance for microbiology, biotechnology and medicine.
- •15. Bacterial cell envelop. The composition and functions of Bacterial Envelope.
- •17. Cell Wall of Gram negative bacteria. The Outer Membrane of Gram-negative Bacteria
- •Characteristics
- •18. Cell Wall-less Forms. Protoplasts. Spheroplasts. L-forms of the bacterium. Mycoplasma
- •19. Appendages structures of bacterial cell. Pili and fimbriae. Properties and functions of pili and fimbriae.
- •Key Concepts:
- •20. The structure and function of the bacterial flagella and axial filaments
- •21. Different arrangements of bacterial flagella. Flagella movement. Correlation of swimming behavior and flagellar rotation. Taxis
- •22. Glycocalyx structure. Capsules, slime Layers. Their functions
- •Vegetative reproduction. Binary fission of Gram positive and Gram negative bacteria. The stage of binary fission. Generation time.
- •Vegetative reproduction. Budding. Multiply fission. The types of grown cycle. Asexual Reproduction of Actinomycetes.
- •Resting cell shape in prokaryotes. Cysts. Endospore. The structure and function.
- •The stage of endospore formation. Germination of endospore.
- •Quorum sensing-social lives of bacteria. Biofilms. Cell-to-cell communication. Signalling molecules.
- •28. Genetic Exchange in Bacteria. Transformation.
- •29. Genetic Exchange in Bacteria. Conjugation.
- •33. Genetic Exchange in Bacteria. Transduction. Types of transduction.
- •31. Systemics and Taxonomy of microorganisms. Classification. Types of taxonomy: numerical, phylogenetic, polyphase. Nomenclature.
- •32) The characteristic features of Archaebacteria. Сlassification of Archaea.
- •34.Unconventional viruses. Defective viruses.
- •35. Diversity of viruses. Classification criteria. Nomenclature of viruses.
- •36 The interaction of the virus with the cell. Reproduction of viruses.
- •37. Bacteriophages. Types of morphology. The chemical composition.
- •38. The types of interaction of phage with the bacterial cell. Lysogenicity.
32) The characteristic features of Archaebacteria. Сlassification of Archaea.
The Archaea constitute a domain of single-celled microorganisms. Archaebacteria ,diverse group of bacteria), sometimes called the archaea and considered a major group unto themselves. Archaebacteria are contrasted with the Eubacteria, from which they differ biochemically in the arrangement of the bases in their ribosomal RNA and in the composition of their plasma membranes and cell walls. There are three major known groups of Archaebacteria: methanogens, halophiles, and thermophiles. The methanogens are anaerobic bacteria that produce methane. They are found in sewage treatment plants, bogs, and the intestinal tracts of ruminants. Ancient methanogens are the source of natural gas. Halophiles are bacteria that thrive in high salt concentrations such as those found in salt lakes or pools of sea water. Thermophiles are the heat-loving bacteria found near hydrothermal vents and hot springs. Many thermophiles are chemosynthetic), using dissolved sulfur or other elements as their energy source and iron as a means of respiration. Archaebacteria emerged at least 3.5 billion years ago and live in environments that resemble conditions existing when the earth was young.
33) Viruses. The composition and structure of the virion.
A virus is a small infectious agent that can replicate only inside the living cells of an organism. Viruses can infect all types of organisms, from animals and plants to bacteria and archaea.Life properties :Opinions differ on whether viruses are a form of life, or organic structures that interact with living organisms. They have been described as "organisms at the edge of life", since they resemble organisms in that they possess genes and evolve by natural selection, and reproduce by creating multiple copies of themselves through self-assembly. Although they have genes, they do not have a cellular structure, which is often seen as the basic unit of life. Viruses do not have their own metabolism, and require a host cell to make new products. They therefore cannot naturally reproduce outside a host cell – although bacterial species such as rickettsia and chlamydia are considered living organisms despite the same limitation. Accepted forms of life use cell division to reproduce, whereas viruses spontaneously assemble within cells. They differ from autonomous growth of crystals as they inherit genetic mutations while being subject to natural selection. Virus self-assembly within host cells has implications for the study of the origin of life, as it lends further credence to the hypothesis that life could have started as self-assembling organic molecules.Structure:Viruses display a wide diversity of shapes and sizes, called morphologies. In general, viruses are much smaller than bacteria. Most viruses that have been studied have a diameter between 20 and 300 nanometres. Some filoviruses have a total length of up to 1400 nm; their diameters are only about 80 nm. Most viruses cannot be seen with an optical microscope so scanning and transmission electron microscopes are used to visualise virions. To increase the contrast between viruses and the background, electron-dense "stains" are used. These are solutions of salts of heavy metals, such as tungsten, that scatter the electrons from regions covered with the stain. When virions are coated with stain (positive staining), fine detail is obscured. Negative staining over comes this problem by staining the background only.A complete virus particle, known as a virion, consists of nucleic acid surrounded by a protective coat of protein called a capsid. These are formed from identical protein subunits called capsomeres. Viruses can have a lipid "envelope" derived from the host cell membrane. The capsid is made from proteins encoded by the viral genome and its shape serves as the basis for morphological distinction., Virally coded protein subunits will self-assemble to form a capsid, in general requiring the presence of the virus genome. Complex viruses code for proteins that assist in the construction of their capsid. Proteins associated with nucleic acid are known as nucleoproteins, and the association of viral capsid proteins with viral nucleic acid is called a nucleocapsid. The capsid and entire virus structure can be mechanically probed through atomic force microscopy.