
- •1.The basic properties of microorganisms. Factors ubiquitous of microorganisms
- •3.Major fields of theoretical and applied Microbiology
- •4.Major Characteristics of Eukaryotes and Prokaryotes
- •6.Sphere -haped bacteria. The variety of forms, their arrangement, examples, a brief description
- •7.Curved-haped bacteria. The variety of forms, their arrangement, examples, a brief description.
- •8.Plazma (cytoplasmic) membrane. Structure. Functions. Destruction of the plasma membrane by antimicrobial agents
- •9.Movement of materials across membranes. Simple diffusion. Facilitated diffusion.Osmosis.
- •10)Movement of materials across membranes. Active transport. Group translocation.
- •12.The Golden age of microbiology. The discoveries of Pasteur and Koch. Their significance for microbiology, biotechnology and medicine.
- •15. Bacterial cell envelop. The composition and functions of Bacterial Envelope.
- •17. Cell Wall of Gram negative bacteria. The Outer Membrane of Gram-negative Bacteria
- •Characteristics
- •18. Cell Wall-less Forms. Protoplasts. Spheroplasts. L-forms of the bacterium. Mycoplasma
- •19. Appendages structures of bacterial cell. Pili and fimbriae. Properties and functions of pili and fimbriae.
- •Key Concepts:
- •20. The structure and function of the bacterial flagella and axial filaments
- •21. Different arrangements of bacterial flagella. Flagella movement. Correlation of swimming behavior and flagellar rotation. Taxis
- •22. Glycocalyx structure. Capsules, slime Layers. Their functions
- •Vegetative reproduction. Binary fission of Gram positive and Gram negative bacteria. The stage of binary fission. Generation time.
- •Vegetative reproduction. Budding. Multiply fission. The types of grown cycle. Asexual Reproduction of Actinomycetes.
- •Resting cell shape in prokaryotes. Cysts. Endospore. The structure and function.
- •The stage of endospore formation. Germination of endospore.
- •Quorum sensing-social lives of bacteria. Biofilms. Cell-to-cell communication. Signalling molecules.
- •28. Genetic Exchange in Bacteria. Transformation.
- •29. Genetic Exchange in Bacteria. Conjugation.
- •33. Genetic Exchange in Bacteria. Transduction. Types of transduction.
- •31. Systemics and Taxonomy of microorganisms. Classification. Types of taxonomy: numerical, phylogenetic, polyphase. Nomenclature.
- •32) The characteristic features of Archaebacteria. Сlassification of Archaea.
- •34.Unconventional viruses. Defective viruses.
- •35. Diversity of viruses. Classification criteria. Nomenclature of viruses.
- •36 The interaction of the virus with the cell. Reproduction of viruses.
- •37. Bacteriophages. Types of morphology. The chemical composition.
- •38. The types of interaction of phage with the bacterial cell. Lysogenicity.
1.The basic properties of microorganisms. Factors ubiquitous of microorganisms
Microorganisms are living organisms that are usually too small to be seen clearly with the naked eye. An organism with a diameter of 1 mm or less are microorganisms and fall into the broad domain of microbiology. Because most of the microorganisms are only a few thousands of a mm in size, they can only be seen with the aid of microscope. The diameter of most of the bacteria does not exceed a thousandth of a millimeter. This value-1 micrometer (micron) or 10.3 mm, and became a "yardstick" microbiologist. Informations fine cell structure shown in nanometers. Dimensions of small cyanobacteria, yeasts and protozoa are within 10 microns. Due to the invisibility of microbes tc the naked eye and the need for special techniques to study them, microbiology was the last of the three major divisions in biology (the other two are botany and zoology) to develop.
At present there is general agreement to include five major groups as microorganisms. The subdivisions are :
1)Bacteriology (Bacteria)
2)Mycology (Fungi)
3)Phycology (Algae)
4)Protozoology (Protozoa)
5)Virology (Viruses)
Microorganisms are present everywhere on earth which includes humans, animals, plants and other living creatures, soil, water and atmosphere. Microbes can multiply in all three habitats except in the atmosphere. Together their numbers far exceed all other living cells on this planet. Microorganisms are relevant to all of us in a multitude of ways.
Many of the properties of microorganisms make them suitable for use in wastewater treatment. A through knowledge of the different physical and chemical functions of microorganisms is essential to an understanding of liquid waste processing technology.
1)Microorganisms use fermentation reactions (protein) to perform complex synthesis under normal temperature and pressure. Hence equipment based on microorganism reactions is simpler than equipment employing physico-chemical reactions and processes, and makes it possible for complex reactions to be driven efficiently.
2)Among living organisms, microorganisms are characterized by rapid proliferation rate, and are capable of rapidly converting substances. (The volume of oxygen consumed by microorganisms is millions of times that required by humans, on a per-unit-weight basis.) .
3)Microorganism's process materials contained in wastewater, which cannot be decomposed by other means.
4)Microorganisms regulate themselves so as to form an organic layer suitable for processing liquid waste. (Self-regulating function).
5)Microorganism reactions are safe, and pose no danger of secondary pollution.
Plasticity of metabolism. In higher plants and animals in relation to changes in metabolism are severely limited the available set of enzymes in the process of individual development of enzymes in them, of course, is changing, however, with a change of environmental conditions, such changes are very minor. Properties of microorganisms differ incomparably greater flexibility. For high ability of bacteria to adapt (adaptation) is absolutely necessary. This is determined by their small size. In the cell, Micrococcus there is only room for a few hundreds of thousands of protein molecules. Therefore, at this time, unnecessary enzymes may not contain cached. Certain enzymes that are essential for processing nutrients synthesized only when the substance appears near the cell. These inducible enzymes may be up to 10% of the total protein in the cell. Thus, cellular regulatory mechanisms in microbes play an important role and substantially manifested more clearly than with other living beings.
2. The role of microorganisms in natural processes and human activities. Beneficial Effects of Microorganisms Microbes are everywhere in the biosphere, and their presence invariably affects the environment that they are growing in. The effects of microorganisms on their environment can be beneficial or harmful or in apparent with regard to human measure or observation. Since a good part of this text concerns harmful activities of microbes (i.e., agents of disease) this chapter counters with a discussion of the beneficial activities and exploitations of microorganisms as they relate to human culture. The beneficial effects of microbes derive from their metabolic activities in the environment, their associations with plants and animals, and from their use in food production and biotechnological processes. Nutrient Cycling and the Cycles of Elements that Make Up Living Systems At an elemental level, the substances that make up living material consist of carbon (C), hydrogen (H), oxygen (O), nitrogen (N), sulfur (S), phosphorus (P), potassium (K), iron (Fe), sodium (Na), calcium (Ca) and magnesium (Mg). The primary constituents of organic material are C, H, O, N, S, and P. An organic compound always contains C and H and is symbolized as CH2O (the empirical formula for glucose). Carbon dioxide (CO2) is considered an inorganic form of carbon. The most significant effect of the microorganisms on earth is their ability to recycle the primary elements that make up all living systems, especially carbon (C), oxygen (O) and nitrogen (N). These elements occur in different molecular forms that must be shared among all types of life. Different forms of carbon and nitrogen are needed as nutrients by different types of organisms. The diversity of metabolism that exists in the microbes ensures that these elements will be available in their proper form for every type of life. The most important aspects of microbial metabolism that are involved in the cycles of nutrients are discussed below. Primary production involves photosynthetic organisms which take up CO2 in the atmosphere and convert it to organic (cellular) material. The process is also called CO2 fixation, and it accounts for a very large portion of organic carbon available for synthesis of cell material. Although terrestrial plants are obviously primary producers, planktonic algae and cyanobacteria account for nearly half of the primary production on the planet. These unicellular organisms which float in the ocean are the "grass of the sea", and they are the source of carbon from which marine life is derived. Decomposition or biodegradation results in the breakdown of complex organic materials to forms of carbon that can be used by other organisms. There is no naturally-occurring organic compound that cannot me degraded by some microbe, although some synthetic compounds such as teflon, styrofoam, plastics, insecticides and pesticides are broken down slowly or not at all. Through the metabolic processes of fermentation and respiration, organic molecules are eventually broken down to CO2 which is returned to the atmosphere. Waste management, whether in compost, landfills or sewage treatment facilities, exploits activities of microbes in the carbon cycle. Organic (solid) materials are digested by microbial enzymes into substrates that eventually are converted to a few organic acids and carbon dioxide. Nitrogen fixation is a process found only in some bacteria which removes N2from the atmosphere and converts it to ammonia (NH3), for use by plants and animals. Nitrogen fixation also results in replenishment of soil nitrogen removed by agricultural processes. Some bacteria fix nitrogen in symbiotic associations in plants. Other Nitrogen-fixing bacteria are free-living in soil and aquatic habitats. Oxygenic photosynthesis occurs in plants, algae and cyanobacteria. It is the type of photosynthesis that results in the production of O2 in the atmosphere. At least 50 percent of the O2 on earth is produced by photosynthetic microorganisms (algae and cyanobacteria), and for at least a billion years before plants evolved, microbes were the only organisms producing O2 on earth. O2 is required by many types of organisms, including animals, in their respiratory processes. The cyanobacterium, Synechococcus, is a primary component of marine and freshwater plankton and microbial mats, The unicellular procaryote is involved in primary production, nitrogen fixation and oxygenic photosynthesis and thereby participates in the cycles of carbon, nitrogen and oxygen. Synechococcus is among the most important photosynthetic bacteria in marine environments, estimated to account for about 25 percent of the primary production that occurs in typical marine habitats.