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.docRT-PCR Master Mix Kit from Applied Biosystems.
10X PCR Buffer II.
Description.
10X PCR Buffer II when diluted 1:10, provides the pH and ionic strength recommended as optimal for PCR optimization.
Specifications.
500 mM KCl, 100 mM Tris-HCl (pH 8.3) in volume of 1.5 mL. Autoclaved.
Environment.
Store the product at -20°C.
MgCl2 Solution.
Description.
MgCl2 Solution contains 25 mM MgCl2 in DEPC treated DI water.
Specifications.
25 mM MgCl2 in DEPC treated DI water.
Environment.
Store the product at -20°C.
RNase Inhibitor.
Description.
50 kDa recombinant enzyme responsible for inhibition of RNase activity. Includes 2000 units (20 Unites/µL) RNase Inhibitor in Storage Buffer (20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 8 mM DTT, 50% (v/v) glycerol). Used with MuLV Reverse Transcriptase.
Specifications.
Sufficient for 100 reverse transcription reactions, 20 µL each.
Environment.
Store at -20oC.
MuLV Reverse Transcriptase.
Description.
Moloney Murine Leukemia Virus Reverse Transcriptase - recombinant RNA dependant DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary cDNA strand. Includes 5000 units (50 Unites/µL) MuLV Reverse Transcriptase in 20 mM Tris-HCl (pH 7.5), 0.1 mM Na2-EDTA, 1 mM DTT, 0.01% Nonidet P40, 50% (v/v) glycerol, DEPC treated DI water. Used with DNA Polymerase, 250 units with Buffer I.
Specifications.
Sufficient for 100 reverse transcription reactions, 20 µL each.
Environment.
Store at -20oC.
DNA Polymerization Mix.
Description.
DNA Polymerization Mix contains 20 mM each dNTP in DEPC treated DI water.
Specifications.
20 mM each dNTP in DEPC treated DI water.
Environment.
Store at -20oC.
Random Hexamers.
Description.
Contains 50 µM short oligonucleotides of random sequence (d(N)6) that anneal randomly to complementary sites on target DNA or RNA.
Environment.
Store at -20oC.