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ПЦР. Материал для студентов / Литература / RT-PCR Master Mix Kit from Applied Biosystems

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RT-PCR Master Mix Kit from Applied Biosystems.

10X PCR Buffer II.

Description.

10X PCR Buffer II when diluted 1:10, provides the pH and ionic strength recommended as optimal for PCR optimization.

Specifications.

500 mM KCl, 100 mM Tris-HCl (pH 8.3) in volume of 1.5 mL. Autoclaved.

Environment.

Store the product at -20°C.

MgCl2 Solution.

Description.

MgCl2 Solution contains 25 mM MgCl2 in DEPC treated DI water.

Specifications.

25 mM MgCl2 in DEPC treated DI water.

Environment.

Store the product at -20°C.

RNase Inhibitor.

Description.

50 kDa recombinant enzyme responsible for inhibition of RNase activity. Includes 2000 units (20 Unites/µL) RNase Inhibitor in Storage Buffer (20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 8 mM DTT, 50% (v/v) glycerol). Used with MuLV Reverse Transcriptase.

Specifications.

Sufficient for 100 reverse transcription reactions, 20 µL each.

Environment.

Store at -20oC.

MuLV Reverse Transcriptase.

Description.

Moloney Murine Leukemia Virus Reverse Transcriptase - recombinant RNA dependant DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary cDNA strand. Includes 5000 units (50 Unites/µL) MuLV Reverse Transcriptase in 20 mM Tris-HCl (pH 7.5), 0.1 mM Na2-EDTA, 1 mM DTT, 0.01% Nonidet P40, 50% (v/v) glycerol, DEPC treated DI water. Used with DNA Polymerase, 250 units with Buffer I.

Specifications.

Sufficient for 100 reverse transcription reactions, 20 µL each.

Environment.

Store at -20oC.

DNA Polymerization Mix.

Description.

DNA Polymerization Mix contains 20 mM each dNTP in DEPC treated DI water.

Specifications.

20 mM each dNTP in DEPC treated DI water.

Environment.

Store at -20oC.

Random Hexamers.

Description.

Contains 50 µM short oligonucleotides of random sequence (d(N)6) that anneal randomly to complementary sites on target DNA or RNA.

Environment.

Store at -20oC.