Подготовка у универсиаде 2012 / hcv_genome_and_protein_processing
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HEPATITIS WEB STUDY
HCV Genome and Protein Processing
Presentation Prepared by:
David Spach, MD
Last Updated: July 18, 2011
Hepatitis
web study
Hepatitis C Genome and Protein Processing
Hepatitis C Genome
HCV Genome
5´ Non-coding Region |
3´ Non-coding Region |
Positive-sense, single strand RNA, ≈ 9,600 base pairs
Hepatitis
web study
Hepatitis C Genome
Regions Coding for Structural & Non-Structural Proteins
HCV Genome
5´ Non-coding Region |
3´ Non-coding Region |
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Genes Coding for |
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Genes Coding for |
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Structural Proteins |
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Non-Structural (NS) Proteins |
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Hepatitis
C Genome
and Nonstructural Genes
HCV Genome
5´ Non-coding Region |
3´ Non-coding Region |
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Genes Coding for |
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Genes Coding for |
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Structural Proteins |
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Non-Structural (NS) Proteins |
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C |
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E1 |
E2 |
p7 |
NS2 |
NS3 |
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A NS4 B |
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A |
NS5 |
B |
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Hepatitis
Hepatitis C Genome
Internal Ribosomal Entry Site (IRES)
HCV Genome
5´ Non-coding Region |
3´ Non-coding Region |
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C |
E1 |
E2 |
p7 |
NS2 |
NS3 |
A NS4 B |
A |
NS5 |
B |
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IRES
Internal Ribosomal Entry Site (IRES)
-Consists of Domains II, III, IV of the 5´ NCR and first 24-40 nucleotides of core region
-IRES involved in processing of polypeptide precursor
Hepatitis
web study
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Hepatitis C Virus |
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Translation |
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HCV Genome |
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5’ NCR |
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3’ NCR |
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Translation
Polyprotein Precursor
C |
E1 |
E2 |
p7 |
NS2 |
NS3 |
A NS4 B |
A |
NS5 |
B |
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Hepatitis
web study
Hepatitis C Virus
Translation
HCV Genome
5’ Non-coding Region |
3’ Non-coding Region |
Translation
C |
E1 |
E2 |
p7 |
NS2 |
NS3 |
A NS4 B |
A |
NS5 |
B |
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Polyprotein Precursor: ≈ 3,000 amino acids
Hepatitis
web study
Hepatitis C Virus
Processing
HCV Genome
Translation
Polyprotein Precursor
C |
E1 |
E2 |
p7 |
NS2 |
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NS3 |
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A |
NS4 |
B |
A |
NS5 |
B |
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Protein Processing |
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Signal Peptidase |
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NS3/4A Protease |
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C |
E1 |
E2 |
p7 |
NS2 |
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NS3 |
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A |
NS4 |
B |
A |
NS5 |
B |
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Hepatitis
web study
Hepatitis C Virus
Protein Processing
HCV Genome
Translation
Polyprotein Precursor
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C |
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E1 |
E2 |
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p7 |
NS2 |
NS3 |
A NS4 B |
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A |
NS5 |
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B |
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Protein Processing |
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Proteins |
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C |
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E1 |
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E2 |
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p7 |
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NS2 |
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NS3 |
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NS4A |
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NS4B |
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NS5A |
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NS5B |
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Hepatitis
web study