Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5946_Библиотеки_им_академика_М_И_Перельмана

cyclotron for irradiation of approximately 2mL of enriched 18O water through the 18O(p,n) 18F nuclear reaction. The radiosynthesis of 18F-FAZA is automatically performed with a synthesis mod­ule. In the rst step of the synthesis, 18F-uoride is separated from 18O water using an anion exchange cartridge. To elute the 18F-uoride absorbed on the resin, a mixture of 0.5mL of a solution of K2CO3 in water (6mg/mL) and 1mL of Kryptox 222 (20mg/mL; Merck) in CH3CN was used. The solution in the reactor is then dried under vacuum at 60 °C for 5 min and then at 120°C for another 5min. Labelling is carried out using 5mg of the precursor dissolved in 1mL of anhydrous dimethyl sulfoxide under stirring at 100°C for 5min. To remove acetyl groups, basic hydrolysis is achieved with 1mL of 0.1N NaOH at 35°C in about 2 min. Neutralization is per­formed by adding 0.5 mL of 0.5 N aqueous NaH2PO4. The reaction mixture has to be com­bined with 2 mL of high-performance liquid chromatography eluent (H2O:EtOH 98:2 [v/v]) and then passed through an Al2O3 cartridge (Waters), where unreacted uorine was adsorbed. Then, the mixture is injected into a semiprepara­tive high-performance liquid chromatography column to separate the reaction product from other impurities. At a ow rate of 4mL/min, the product has a retention time of about 34 min, detected with an ultraviolet detector in series with a Geiger–Müller radiodetector. The col­lected radiopharmaceutical has to be ltered using a 0.22-μm sterile lter and then diluted with 0.9% NaCl in order to have 12mL.