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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5896_Библиотеки_им_академика_М_И_Перельмана
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160 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Fig. (105a). Canvas painting of Tomato illustrated by Patanjali Research Foundation, Haridwar, India.
Fig. (105b). HPTLC fingerprinting of Tomato: S: Lycopene, T: Tomato fruit.
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 161
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Solanum nigrum L. (Common Name: Makoy)
Plant Part: Fruit.
Solvent System: Toluene: ethyl acetate: formic acid (10: 10: 2 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Sample Solution Preparation: 500 mg of the sample dissolved in 10 mL
methanol, sonicated and centrifuged. 10 μL of the solution was applied on a TLC
plate.
Medicinal Value: Used as tonic, laxative, appetite stimulant, for treating asthma
and liver disease (3).
Conclusion: HPTLC fingerprint of Makoy represents bands of phytochemicals at
254 nm and 366 nm (Fig. 106a, b).
Fig. (106a). Canvas painting of Makoy illustrated by Patanjali Research Foundation, Haridwar, India.
162 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Fig. (106b). HPTLC fingerprint of Makoy: T: Makoy fruit.
Solanum surattense Burm. F. (Common Name: Chhoti Kateli)
Plant Part: Aerial part.
Solvent System: Ethyl acetate: toluene: formic acid (5: 5: 0.5 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Standard Solution Preparation: 10 mg of apigenin and caffeic acid was
dissolved in 10 mL of methanol. 10 μL of the standard solution was applied on the
TLC plate.
Sample Solution Preparation: 500 mg sample dissolved in 10 mL methanol,
sonicated and centrifuged.10 μL of the solution was applied on a TLC plate.
Medicinal Value: Used to treat fever, cough, sore throat, and urinary track
disorders (1).
Conclusion: HPTLC image confirms the identification of marker apigenin and
caffeic acid at Rf 0.45 and 0.52 with respect to standards in Chhoti kateli (Fig.
107a, b).
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 163
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Fig. (107a). Canvas painting of Chhoti Kateli illustrated by Patanjali Research Foundation, Haridwar, India.
Fig. (107b). HPTLC fingerprint of Chhoti kateli: S1: Apigenin, T: Chhoti kateli, S2: Caffeic acid.
164 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Swertia chirayita (Roxb.) H.Karst (Common Name: Chirayita)
Plant Part: Whole plant.
Solvent System: Chloroform: methanol: formic acid (7: 3: 0.5 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Standard Solution Preparation: 10 mg of lancerin was dissolved in 10 mL of
methanol. 10 μL of the standard solution was applied on a TLC plate.
Sample Solution Preparation: 500 mg of sample dissolved in 10 mL methanol,
sonicated and centrifuged. 4 μL of the solution was applied on a TLC plate.
Medicinal Value: Used to treat fever and intestinal worms; also have antimalarial property (2).
Conclusion: HPTLC fingerprint of Chirayita shows the presence of lancerin in
the sample at 0.56 Rf (Fig. 108a, b).
Fig. (108a). Canvas painting of Chirayita illustrated by Patanjali Research Foundation, Haridwar, India.
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 165
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Fig. (108b). HPTLC fingerprint of Chirayita: S1: Lancerin, T1: Chirayita whole plant.
Syzygium aromaticum (L.) Merr. & L.M.Perry (Common Name: Clove)
Plant Part: Buds.
Solvent System: Ethyl acetate: toluene: formic acid (10: 4: 3 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Sample Solution Preparation: 500 mg sample was dissolved in 10 mL
methanol, sonicated and centrifuged. 10 μL of the solution was applied on a TLC
plate.
Medicinal Value: Useful in the treatment of vomiting flatulence, nausea, liver,
bowel diseases and stomach disorders (3).
Conclusion: HPTLC fingerprint of Clove shows a specific banding pattern at 254
nm and 366 nm (Fig. 109a, b).
166 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Fig. (109a). Canvas painting of Clove illustrated by Patanjali Research Foundation, Haridwar, India.
Fig. (109b). HPTLC fingerprint of Clove: T: Clove buds.
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 167
https://t.me/med1917
Terminalia arjuna (Roxb. ex DC.) Wight & Arn (Common Name: Arjun)
Plant Part: Bark.
Solvent System: Ethyl acetate: toluene: formic acid: acetic acid (5: 3: 0.5: 1
v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Standard Solution Preparation: 10 mg of arjunic acid was dissolved in 10 mL
of methanol. 10 μL of the standard solution was applied on a TLC plate.
Sample Solution Preparation: 500 mg sample dissolved in 10 mL methanol
sonicated and centrifuged.10 μL of the solution was applied on a TLC plate.
Derivatizing Reagent: Anisaldehyde sulfuric acid.
Medicinal Value: The plant has cardiovascular, antioxidant and anti-
inflammatory properties (1).
Conclusion: HPTLC fingerprint of Arjun shows the presence of arjunic acid at Rf
0.70 with respect to the standard (Fig. 110a, b).
Fig. (110a). Canvas painting of Arjun illustrated by Patanjali Research Foundation, Haridwar, India.
168 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Fig. (110b). HPTLC fingerprint of Arjun: S1: Arjunic acid, T1: Arjun bark.
Terminalia bellirica (Gaertn.) Roxb (Common Name: Bahera)
Plant Part: Fruit.
Solvent System: Ethyl acetate: toluene: formic acid (5: 2: 1.5 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Standard Solution Preparation: 10 mg of gallic acid was dissolved in 10 mL of
methanol. 20 μL of the standard solution was applied on a TLC plate.
Sample Solution Preparation: 1000 mg sample dissolved in 10 mL methanol
sonicated and centrifuged. 6 μL of the solution was applied on a TLC plate.
Medicinal Value: Used in skin disorder, fever, indigestion and cough.
Conclusion: HPTLC image confirms the identification of signature marker gallic
acid at Rf 0.61, with respect to standard (Fig. 111a, b).
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 169
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Fig. (111a). Canvas painting of Bahera illustrated by Patanjali Research Foundation, Haridwar, India.
Fig. (111b). HPTLC fingerprint of Bahera: S: Gallic acid, T: Bahera fruit.
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