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Файл:Ординатура / Хирургия / Библиотека им академика М.И. Перельмана / Книга_5375_Библиотеки_им_академика_М_И_Перельмана
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120 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Fig. (78b). HPTLC fingerprint of Bitter gourd: T: Bitter gourd fruit.
Moringa oleifera Lam (Common Name: Sahjan)
Plant Part: Leaves.
Solvent System: Toluene: ethyl acetate: formic acid (5: 5: 1 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Sample Solution Preparation: 1000 mg of the sample dissolved in 10 mL
methanol, sonicated and centrifuged. 10 μL of the solution was applied on a TLC
plate.
Medicinal Use: The plant is used as cardiac and circulatory stimulant, possess
antitumor, antipyretic, anti-inflammatory, antiulcer, antispasmodic, diuretic,
antioxidant, antibacterial and antifungal activities (3).
Conclusion: HPTLC fingerprint represents specific bands of phytochemical
molecules of Sahjan at 254 nm and 366 nm (Fig. 79a, b).
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 121
https://t.me/med1917
Fig. (79a). Canvas painting of Sahjan illustrated by Patanjali Research Foundation, Haridwar, India.
Fig. (79b). HPTLC fingerprint of Sahjan: T: Sahjan leaves.
122 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Murraya paniculata (L.) Jack (Common Name: Gandhani)
Plant Part: Leaves.
Solvent System: Ethyl acetate: toluene: formic acid (5: 5: 0.5 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Sample Solution Preparation: 1000 mg of the sample dissolved in 10 mL
methanol, sonicated and centrifuged. 3 μL of the solution was applied on a TLC
plate.
Medicinal Value: Used to treat fever, cough sore throat, dysentery and diarrhoea.
Conclusion: Fingerprint of Gandhani showing specific patterns of bands at 254
nm and 366 nm (Fig. 80a, b).
Fig. (80a). Canvas painting of Gandhani illustrated by Patanjali Research Foundation, Haridwar, India.
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 123
https://t.me/med1917
Fig. (80b). HPTLC fingerprint of Gandhani: T: Gandhani leaves.
Myristica fragrans Houtt (Common Name: Mace)
Plant Part: Aril.
Solvent System: Toluene: ethyl acetate formic acid (8: 2: 0.3 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Sample Solution Preparation: 500 mg sample dissolved in 10 mL of methanol,
sonicated and centrifuged. 10 μL of the solution was applied on a TLC plate.
Medicinal Value: Useful in clearing throat, cough, asthma, throat pain, irritable
bowel syndrome and diarrhoea (2).
Conclusion: HPTLC Fingerprint of Mace showing a specific pattern of bands at
254 nm (Fig. 81a, b).
124 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Fig. (81a). Canvas painting of Mace illustrated by Patanjali Research Foundation, Haridwar, India.
Fig. (81b). HPTLC fingerprint of Mace: T: Mace aril.
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 125
https://t.me/med1917
Myristica fragrans Houtt (Common Name: Jaiphal)
Plant Part: Fruit.
Solvent System: Toluene: ethyl acetate formic acid (7: 3: 1 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Sample Solution Preparation: 1000 mg sample dissolved in 10 mL of methanol,
sonicated and centrifuged. 10 μL of the solution was applied on a TLC plate.
Medicinal Value: Useful in clearing throat, cough, asthma, throat pain, irritable
bowel syndrome and diarrhoea (2).
Conclusion: HPTLC fingerprint of Nutmeg represents bands of phytochemicals
at 254 nm, fluorescent bands at 366 nm and blue, bluish violet bands under white
light after derivatization (Fig. 82a, b).
Fig. (82a). Canvas painting of Jaiphal illustrated by Patanjali Research Foundation, Haridwar, India.
126 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Fig. (82b). HPTLC fingerprint of Nutmeg: T: Nutmeg fruit.
Nardostachys jatamansi (D.Don) DC (Common Name: Jatamansi)
Plant Part: Roots.
Solvent System: Toluene: ethyl acetate formic acid (8: 2: 0.3 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Sample Solution Preparation: 1000 mg sample dissolved in 10 mL methanol,
sonicated and centrifuged. 10 μL of the solution was applied on a TLC plate.
Medicinal Value:Useful in inducing sound sleep, improving intelligence and
reducing anxiety (2).
Conclusion: HPTLC photograph at 254 nm and 366 nm represents a specific
band pattern for Jatamansi in given chromatographic conditions (Fig. 83a, b).
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 127
https://t.me/med1917
Fig. (83a). Canvas painting of Jatamansi illustrated by Patanjali Research Foundation, Haridwar, India.
Fig. (83b). HPTLC fingerprint of Jatamansi: Jatamansi roots.
128 Chemical Fingerprints of Medicinal Plants - HPTLC Profiling Acharya Balkrishna
https://t.me/med1917
Nymphaea nouchali Burm. F. (Common Name: Blue Lotus)
Plant Part: Flower.
Solvent System: Toluene: ethyl acetate formic acid (7: 3: 1 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck)
254
Sample Solution Preparation: 1000 mg sample dissolved in 10 mL methanol,
sonicated and centrifuged. 10 μL of the solution was applied on a TLC plate.
Medicinal Value: Useful in bleeding disorders, body ache, and skin boils and
provides relief from burning sensations (1).
Conclusion: HPTLC fingerprint of blue lotus shows spots of phytochemicals
under UV and white light. Fluorescent bands observed at 366 nm and blue and
purple bands under white light after derivatization (Fig. 84a, b).
Fig. (84a). Canvas painting of Blue lotus illustrated by Patanjali Research Foundation, Haridwar, India.
Medicinal Plants Chemical Fingerprints of Medicinal Plants - HPTLC Profiling 129
https://t.me/med1917
Fig. (84b). HPTLC fingerprint of Blue lotus: T: Blue lotus flower.
Ocimum basilicum L. (Common Name: Basil)
Plant Part: Leaves.
Solvent System: Ethyl acetate: toluene: formic acid (10: 9: 1 v/v/v).
Adsorbent: TLC Silica gel 60F
(Merck).
254
Sample Solution Preparation: 1 g of sample dissolved in 10 mL methanol:
water (60: 40 v/v), sonicated and centrifuged. 8 μL solution was applied on the
TLC plate.
Medicinal Value: Plant has antibacterial, antifungal and insecticidal activities (2).
Conclusion: HPTLC fingerprint shows bands of phytochemical components at
254 nm, 366 nm and under white light after derivatization (Fig. 85a, b).
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