Использование новых штаммов молочнокислых бактерий в птицеводстве в качестве основы пробиотиков. Рекомендация
.pdf
11
4
+ ' %
5, |
|
|
" ! |
& |
& |
0 %, % |
/ |
|
|
|
& |
|
|
|
|
|
|
|
|
|
1 |
1- |
!& |
! % ! % % |
75 |
73 |
97,33 |
|
Lactobacillus acidophilus (1 .74/17) |
|
|
|
||
|
|
|
|
|
|
|
2 |
2- |
!& |
! % ! % !( % |
75 |
70 |
93,33 |
|
Streptococcus lactis (1 .31/11) |
|
|
|
||
33- !& Lactobacillus acidophilus
(1 .74/17)+Streptococcus |
lactis |
75 |
75 |
100,0 |
(1 .31/11) |
|
|
|
|
|
|
|
|
|
4 4- % ! !& |
- |
75 |
64 |
85,33 |
(2 ) |
|
|
|
|
12
|
|
|
|
|
|
|
|
|
|
|
5 |
|
|
|
|
+ ' % |
|
|
|
|
|||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
+ |
|
|
|
|
|
+ |
|
|
" ! |
G |
|
2 ) |
- |
|
+ |
|
% |
|
||
|
|
|
|
( ) |
( ) |
|
|
|
|
|
( |
|
|
|
( ) |
|
|
|
|
10 ! |
' 20 ! |
|
30 ! |
% |
|
|
|
|
|
|
|
( ) |
( ) |
|
( ) |
|
||
|
|
|
|
|
|
|
|
|
|
|||
|
|
|
|
|
|
|
|
|
|
|
|
|
1-!& |
Lactobacillus |
75 |
|
6405,0 |
13357,5 |
|
94,9 |
166,7 |
|
263,5 |
95,0 |
|
acidophilus (1 .74/17) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
2-!& |
Streptococcus |
75 |
|
6870,0 |
14130,0 |
|
95,0 |
165,5 |
|
266,8 |
98,0 |
|
lactis (1 .31/11) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
3-!& |
Lactobacillus aci- |
75 |
|
6780,0 |
20003,13 |
|
98,0 |
173,1 |
|
278,3 |
100,0 |
|
dophilus (1 .74/17) + Strepto- |
|
|
|
|
|
|
|
|
|
|
|
|
coccus lactis (1 .31/11) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4-% ! |
75 |
|
6982,5 |
10875,0 |
|
91,2 |
156,1 |
|
237,7 |
85,33 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
13
6 + ' ' & $, ! %
& ! % ! % &
|
4 |
|
G ' 10 - |
G ' 20 - |
G ' 30 ! % ! % - |
||||||||||||
5 |
|
|
|
|
|
|
! % ! % - |
! % ! % - |
|
|
|
|
|||||
2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
"- |
|
G |
|
G |
|
"- |
G |
G |
" |
G |
G |
" |
G |
|
G |
|
|
|
|
$ - |
|
- |
|
|
$ - |
- |
- |
$ - |
- |
0 |
$ |
|
|
|
|
0 - |
|
|
|
|
|
|
0 - |
|
|
|
|
|
( %) |
13 |
|
13 |
|
( |
|
13 |
|
|
|
|
( |
13 |
|
|
13 |
13 |
|
|
|
|
|
%) |
|
|
|
13 |
|
%) |
|
13 |
0 - |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
( |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
%) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
|
|
|
|
1- ! |
, !& 1 Lactobacillus acidophilus (1 .74/17) |
|
|
|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
1. |
50 |
|
2,625440 |
|
185050 |
|
68 |
|
2,817100 |
19100 |
69 |
2,853600 |
21500 |
75 |
2,666415 |
|
22900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
2. |
48 |
|
2,398340 |
|
179006 |
|
66 |
|
2,811720 |
18000 |
63 |
2,671500 |
21300 |
75 |
2,535859 |
|
22800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
3. |
52 |
|
2,540790 |
|
182007 |
|
66 |
|
2,896900 |
18300 |
67 |
2,970700 |
22100 |
78 |
2,593100 |
|
22800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4. |
69 |
|
3,780990 |
|
19000 |
|
71 |
|
3,109050 |
19200 |
74 |
2,447500 |
22350 |
70 |
2,837980 |
|
22900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
5. |
54 |
|
2,477160 |
|
18400 |
|
65 |
|
2,894880 |
20000 |
66 |
2,940000 |
22100 |
75 |
2,569800 |
|
22000 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
6. |
60 |
|
2,655060 |
|
18350 |
|
68 |
|
2,350760 |
19000 |
69 |
2,053500 |
23200 |
75 |
2,688100 |
|
23500 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
7. |
55 |
|
2,423100 |
|
19400 |
|
60 |
|
2,302880 |
20100 |
60 |
3,325200 |
22300 |
76 |
2,665285 |
|
22500 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
8. |
60 |
|
2,474640 |
|
18450 |
|
70 |
|
2,937000 |
19200 |
68 |
2,946790 |
21900 |
79 |
2,664350 |
|
24900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
9. |
60 |
|
2,383830 |
|
19000 |
|
62 |
|
2,990610 |
19300 |
63 |
2,780520 |
23200 |
72 |
2,450485 |
|
24800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
10. |
50 |
|
2,859760 |
|
18000 |
|
67 |
|
3,184780 |
19150 |
66 |
3,112600 |
22200 |
78 |
2,576205 |
|
24800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
C . 1- |
55,8 |
|
2,461911 |
|
18,520 |
|
66,3 |
2,829568 |
19,135 |
66,5 |
2,810191 |
21260 |
71,4/127,9% |
2,838000/110,08% |
|
23490/119,1% |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
||
|
|
2- ! |
, !& 1 Streptococcus lactis (1 .31/11) |
|
|
|
|
|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
11. |
66 |
|
2,368800 |
|
19300 |
|
65 |
|
2,521950 |
20200 |
66 |
2,542800 |
22300 |
68 |
2,532375 |
|
20800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
12. |
63 |
|
2,317100 |
|
19400 |
|
68 |
|
2,842560 |
18800 |
68 |
2,447500 |
23200 |
70 |
2,645030 |
|
21200 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
13. |
62 |
|
2,232750 |
|
18800 |
|
66 |
|
2,223800 |
21000 |
65 |
2,222600 |
23500 |
69 |
2,223200 |
|
22450 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
14. |
68 |
|
2,729210 |
|
18500 |
|
72 |
|
2,605680 |
22300 |
73 |
2,714250 |
23500 |
70 |
2,659965 |
|
23200 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
15. |
66 |
|
2,910770 |
|
18800 |
|
66 |
|
2,762100 |
22500 |
78 |
2,850700 |
22300 |
85 |
2,806400 |
|
22100 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
16. |
62 |
|
2,149480 |
|
19500 |
|
69 |
|
2,732740 |
21600 |
80 |
2,571360 |
23000 |
79 |
2,652050 |
|
22500 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
17. |
56 |
|
2,483700 |
|
18600 |
|
66 |
|
2,353150 |
22100 |
84 |
2,457500 |
23400 |
80 |
2,405325 |
|
22400 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
18. |
58 |
|
2,149480 |
|
18900 |
|
66 |
|
2,752360 |
21900 |
85 |
2,752400 |
23600 |
64 |
2,752380 |
|
23100 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
19. |
56 |
|
2,483700 |
|
19900 |
|
65 |
|
2,367800 |
22400 |
86 |
2,072700 |
22200 |
69 |
2,170250 |
|
22800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
20. |
60 |
|
2,090780 |
|
19800 |
|
68 |
|
2,448120 |
22800 |
78 |
3,505740 |
22990 |
75 |
3,476930 |
|
22900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
C . 1- |
61,7 |
|
2,730355 |
|
19150 |
|
67,1 |
2,361026 |
21,560 |
69,3 |
2,616039 |
22090 |
72,9/118,15% |
2,632532/103,71% |
|
22,345/116,6% |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
+ 6
14
|
3- ! , |
!& 1 Lactobacillus acidophilus (1 .74/17) |
Streptococcus lactis (1 .31/11) |
|
|||||||||||||
1 |
|
2 |
|
3 |
4 |
|
5 |
|
6 |
7 |
8 |
9 |
|
10 |
11 |
12 |
13 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
21 |
|
68 |
|
2.570850 |
18100 |
|
61 |
|
2.781030 |
20100 |
62 |
2.949000 |
|
20800 |
85 |
2.666415 |
22900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
22 |
|
65 |
|
2.973120 |
18200 |
|
67 |
|
2.541400 |
20800 |
65 |
2.545400 |
|
22100 |
85 |
2.535855 |
228000 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
23 |
|
65 |
|
1.999200 |
17900 |
|
69 |
|
1.962000 |
19500 |
67 |
2.557800 |
|
23100 |
78 |
2.593100 |
22800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
24 |
|
60 |
|
3.051750 |
18500 |
|
64 |
|
2.899850 |
19600 |
69 |
2.749600 |
|
22500 |
80 |
2.837980 |
22900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
25 |
|
64 |
|
2.682460 |
17900 |
|
63 |
|
3.021800 |
19400 |
75 |
2.656800 |
|
21900 |
85 |
2.566980 |
22000 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
26 |
|
65 |
|
2.374020 |
18600 |
|
67 |
|
2.513930 |
19700 |
62 |
2.735600 |
|
22800 |
85 |
2.688100 |
23500 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
27 |
|
69 |
|
2.437840 |
18700 |
|
65 |
|
2.555800 |
19800 |
67 |
2.666640 |
|
23400 |
86 |
2.665285 |
22500 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
28 |
|
61 |
|
2.674240 |
18800 |
|
60 |
|
2.432500 |
19500 |
65 |
2.881200 |
|
22600 |
89 |
2.664350 |
24900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
29 |
|
65 |
|
2.915600 |
18700 |
|
68 |
|
2.689050 |
19400 |
60 |
2.522000 |
|
23600 |
82 |
2.450485 |
24800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
30 |
|
66 |
|
2.435360 |
19000 |
|
62 |
|
2.006950 |
19800 |
69 |
2.428010 |
|
22900 |
88 |
2.576205 |
24800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
C . 1- |
|
61.8 |
|
2.750222 |
18440 |
|
62.6 |
|
2.560321 |
19760 |
65.1 |
2.669205 |
|
22570 |
71.4/123.2% |
2.838000/110.08% |
23490/127.3% |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
% |
! |
!& |
(2 ) ' |
|
|
|
|
|
|
|||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
31 |
|
50 |
|
2.383830 |
18000 |
|
60 |
|
3447500 |
18500 |
68 |
2.949000 |
|
19100 |
65 |
2.666415 |
20800 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
32 |
|
48 |
|
2.526310 |
17700 |
|
55 |
|
2.290860 |
17900 |
66 |
2.545400 |
|
18000 |
65 |
2.535859 |
22100 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
33 |
|
50 |
|
2.628400 |
18000 |
|
59 |
|
2.428010 |
18200 |
66 |
2.557800 |
|
18300 |
68 |
2.593100 |
23100 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
34 |
|
67 |
|
2.926360 |
17800 |
|
66 |
|
2.926360 |
19000 |
71 |
2.749600 |
|
19200 |
68 |
2.837980 |
22500 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
35 |
|
54 |
|
2.477160 |
18100 |
|
50 |
|
2.587200 |
18400 |
65 |
2.656800 |
|
20000 |
67 |
2.566980 |
21900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
36 |
|
60 |
|
2.640600 |
17900 |
|
65 |
|
2.230560 |
18350 |
68 |
2.735600 |
|
19000 |
65 |
2.688100 |
22800 |
|
|
|
|
|
|||||||||||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
37 |
|
55 |
|
2.666930 |
17800 |
|
63 |
|
2.671040 |
19400 |
60 |
2.666640 |
|
20100 |
68 |
2.665285 |
23400 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
38 |
|
60 |
|
2.447500 |
18200 |
|
60 |
|
3.008600 |
18450 |
70 |
2.881200 |
|
19200 |
67 |
2.664350 |
22600 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
39 |
|
60 |
|
2.378970 |
18000 |
|
59 |
|
2.347200 |
19000 |
62 |
2.522000 |
|
19300 |
65 |
2.450485 |
23600 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
40 |
|
50 |
|
2.724400 |
17900 |
|
60 |
|
2.825680 |
18000 |
67 |
2.428010 |
|
19150 |
66 |
2.576205 |
22900 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
C . 1- |
|
55.4 |
|
2.579746 |
17940 |
|
59.7 |
|
2.676301 |
18520 |
66.3 |
2.669181 |
|
19.135 |
66.4/117.8% |
2.624479/101.73% |
19760/110.1% |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
! 61,7 72,9 ( 118,15%); & $ ! & % 2.632532 2730355 ( 103,71%) 19150 22.345 ( 116,6%); % ! -68,8 82,3 ( 123,2%), & $ -! & % 2.750960 2.838000 ( 110,08%) 1844023490 ( 127,3%); % ! -55,4 66,4 ( 110,73 %) & $ 2,624479 ( 101,73%) & 17940 19760 ( 110,1%).
' , !& $ ! (
, & & ' ( %
' , !( !& ( % ! 1 & .
2. ! 2 % 22 <
C ! C % -& (2 , 1959; G , * ! ,1957; * ,1958; &, C , , 1965; C , ,1966; H , ! % , 1968) %' -! % ! ' : Pichia fermentans Lodder C #-296 ( -), Saccharomyces cerevisiae Hansen C #-1168 (1 O – ), Zygofabospora marxiana (Hansen) Kudriavzev, C #-832 ( ), Debariomyces disporus (Beijerinck) Dekker C #-1034 ( ), Zygosaccharomyces bailii (Linder) Guilliermondii C 3-853 ( ), Saccharomyces carlsbergensis rasse Carlsberg C #-1173 ( ' ), (2 , 1959) E. coli 1 113-3 ( ) (Davis, Mingioli, 1950).
+ & -! % ! % ! 1 1 && , ! (1 C C - 361), ! (C C- 362), ' ! (C C - 363), ! (C C-469), ' ! (C C - 470), ! (C C - 471), ! (C C - 824), ! (C C - 830), ! (C C - 831), ! ! (C C - 836), !& ' ! % ! ! Q3 000 .
+ % !& ! ( ,, 1981, 1986).
15
1.+ ! % ! % -( ( -
) 1 ( 1 & ! ' & 1:1 (1 ! % ! % 1 ! ).
2.0 % ! % ! ! !1 % !- 1 % 1 ! ! 6000 10-
|
12%. + ! & )- |
|
!( & . |
3. |
C !1 ' %& ( % * #-1,2. |
4. |
" ( ! 1 , |
|
( $ ! ' , &- |
|
, ' . |
5.C !1 ( ( %6-7 & ! - .
4.2
1.+ & ( «- » ! & ' 30 3 ' %.
2. ! % ' ! ,% ! ' .
3. 3 & ' ! % ! % Lactobacillus acidophilus (1 .74/17) ! 1000 (1 . . . 1 ! % ! % ) 1 ;
4. + ' ! % ! Streptococcus lactis (1 .31/11) ' & -Lactobacillus acidophilus 1 ;
5.& % ' , & !( !& ( % - ! 1 & .
6.+ % & !-
& & |
$ 13 1 - |
|
( % 0 ). N ' %1 !&- |
% ! ( 10,1%, $
8,35%, 9% %1 ) ( % |
! . |
|
7. = % !& |
! - |
|
! 5,4%, $ 2,18 % 17,2 %, |
! |
|
! 0,35%, $ 1,98%, 6,5 %, |
% |
|
! % ' ! 10,1 %, $ 8,35% 9% %1 .
16
8. C '! % & %! ! 93,33-97,33% 100%( % , $ ' % 85,33 %.
9. * % ! U– ! !& 10,8%, UU- ! - 12,2% UUU– ! -17% %1 (% ! , & ' % -& . + , -1000 1 -.
10.W ' & ) , ) &-, ' %$! % ( ' .
11. + , ' ' -& , ! ) % ! -, ! ! ' 3 % ! (050092, . , . , 8).
O !
1.C. . – ' - . – Q % : -1966.- 0.3-5.
2.C. ., + 4. 3.- & -& ! . – .: - ! C34 4. – 1959. – . 20. – 0.- 40-41.
3.C. .- & ! . – .: - 1969.- 308 .
4.C 3. ., 4. C. – & &-' ! E. coli. – .: - -. – 1966.- .XXXV. – . 3. – 0. 466471.
5." ' C. C.- & ! . - .: - 1974. – 118 .
6.* C. 0.- & & -C12 . Y % ( - & . – .: - 1958. – 42 .
7..- & ' /
.+ . – .: - 2002. - 57. – .27-28.
8.& Z. 3. C O. +., N. ". - 4 ! % ! & ' . – .: - . + . - 1965. . 1.- .5.- 0. 554-558.
9.=.4. – & .– : -1960. –352 . 10. =. 4., 3 2. . – & !
%' . – .: - 4' . ! . - 1975.- 389 .
17
11.2 =. 3.- & .- .: - 4' . 3 000 .- 1959.- 379 .
12.0! . . – 4'!& ' ! ! & . . . . / . ! . - .: - 1978. -18 .
13. ., 2.- C & ! ' ' - ( ( ! . - - : - 1981.-12 .
14. ., 2. –3 ( -«# » ) – (" ! " 000 30 ( 1986 . 5 432-5).
15.! . .– & . - :- 2002.- 164 .
16.G 0. ., * ! =. 3.- # ) & 1 & -C12 – .:- . .- 1957.- . XXVI. – . 4. – 0. 609 - 613.
17.H 3. ., ! % 4. +.- 2 ' ! C -1 ! % . – .: - . – 1968.- . XXVII.- .3.- 0. 433 - 439.
18.Davis B., Mingoli E. -Mutans of Escherihia coli requirig methionine of vitamin B.“Bacteriol.”.–1950.-60.-54,17.
18
Подписано в печать 28.07.2014 г. Объем 1,25 усл. печ. л.. Бумага офсетная. Печать цифровая. Тираж 100 экз.
Отпечатано в типографии «Нур-Принт». Тел.: 98-64-02, 308-25-46 e-mail: nur-print@mail.ru
