Ординатура / Офтальмология / Английские материалы / Retinal Degeneration Disease_Hollyfield, Anderson, LaVail_1999

Figure 60.1. Rod and cone death induced by hyperoxia. Blue is bisbenzamide labeling of DNA, showing (A-D) the major layers of the retina (i – inner nuclear layer, o - outer nuclear layer). Red is TUNEL-labeling. Green in E - H is PNA labeling of cone sheaths. A: Young adult C57BL/6J retina, kept in room air (normoxic control). B, C, D: Young adult C57BL/6J retina after 14d, 21d and 35d in hyperoxia. These images show the most affected region of retina, at each exposure time. TUNEL labeling is restricted to the ONL up to 14d of exposure, but labeling of the INL is evident at 21d and 35d. E-H: Regions at the outer surface of the ONL. Occasional TUNEL+ nuclei were closely associated with cone sheaths (labeled green with PNA lectin). F is part of E, at higher power. The scale bar in E represents 10 mm in E, and 5 mm in F, G, H.

Figure 63.4. Translocation of myc-tagged arrestin-GFP fusion in transgenic tadpoles. Confocal images were obtained from cryosections of xAr-myc-GFP transgenic tadpoles that were dark-adapted overnight (DA) or exposed to light for 45 min (LA 45 min). The endogenous fluorescence of the GFP protein was imaged.

Figure 63.6. Confocal images of xAr-4K Æ Q-GFP translocation in rod photoreceptors. Transgenic tadpoles were dark-adapted overnight (DA), light adapted for 50 min (LA 50 min), or light adapted for 240 min (LA 240 min). Images show the endogenous fluorescence of the GFP in the fusion protein.