HSV Cytopathic Effect: Advanced Changes

Fig. 1.5  This cell culture infected with a laboratory (HSV-1) virus strain shows advanced changes: All remaining cells show the virus cytopathic effect (cell swelling and rounding, arrowheads); moreover, in the cell monolayer, cell bursting and disappearance have resulted in cell-denuded areas (arrows)

Fig. 1.6  This HSV lesion in the living human corneal epithelium shows closely packed swollen/rounded cells, some appearing dull (black arrowhead) and others bright (white arrowhead); in the multilayered epithelium, cell bursting and disappearance have not resulted in cell-denuded areas yet (cf. Fig. 1.7, opposite page)

a

b

Fig. 1.7  a–b The appearance of these two HSV lesions in the living human corneal epithelium is the result of cell swelling (arrowheads), bursting, and disappearance. In places, the loss of substance seems to have reached the level of the epithelial basement membrane (arrows indicate apparently cell-denuded areas)

Comment

Level differences can be visualized with fluorescein sodium (cf. Fig.1.9).