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Ординатура / Офтальмология / Английские материалы / Atlas of Confocal Laser Scanning In-vivo Microscopy in Opthalmology - Principles and Applications in Diagnostic and Therapeutic Ophtalmology_Guthoff, Baudouin, Stave_2006

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5.5 Stroma

83

 

 

a

c

Fig. 5.75 Corneal wound healing: recently healed ulcer. a Activated corneal epithelium with reflective cells. b Numerous activated keratocytes in the ante-

b

d

rior stroma below Bowman’s layer. c, d Multiple inflammatory cells: dendritic cells

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Chapter 5 Confocal Laser Scanning In Vivo Microscopy

 

 

a

b

Fig. 5.76 Penetrating keratoplasty. a Oblique section showing the corneal graft: irregular and hyperreflec-

tive epithelium and activated stroma. b Dendritic cells at the edge of the corneal graft

a

b

Fig. 5.77 Penetrating keratoplasty. Immune ring at the edge of a corneal graft in a case of epithelial rejec-

tion, with precipitates (reflective dots) in the corneal epithelium

5.5 Stroma

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a

b

Fig. 5.78 Penetrating keratoplasty. a Periphery of the corneal graft: inflammatory cells on both sides. b Corneal suture penetrating the basal corneal epithelium

a

b

Fig. 5.80 Same patient as in Fig. 5.79: confocal microscopy images of a furrow. Note the presence of corneal epithelial cells located very deeply in the bed of the incision

Fig. 5.79 Refractive surgery: slit-lamp photograph of a radial keratotomy performed 15 years previously

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Chapter 5 Confocal Laser Scanning In Vivo Microscopy

 

 

a

b

Fig. 5.81 Refractive surgery: photorefractive keratectomy. a Corneal haze 3 years after photorefractive keratectomy. Activated corneal epithelial cells are ab-

normally hyperreflective. b At a deeper level, fibrosis of the epithelial basement membrane, activated keratocytes, and hyperreflective deposits

a

b

 

Fig. 5.82 Refractive surgery: photorefractive

 

keratectomy. a Slit-lamp photograph 4 weeks after

 

photorefractive surgery; appearance of grade 1+

 

corneal haze. b Confocal in vivo microscopy image

 

4 weeks after photorefractive surgery; grade 1+

 

haze. Keratocytes with activated nuclei and cyto-

 

plasmic processes produce a network appearance.

 

c Same patient. Hyperreflective keratocytes with

 

less readily identifiable individualized nuclei and

c

fewer cytoplasmic processes, at the level of a zone

of possible keratocyte apoptosis

 

5.5 Stroma

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a

Fig. 5.83 Refractive surgery: laser-assisted in situ keratomileusis (LASIK). a Slit-lamp photograph of a 25-year-old woman after LASIK surgery. Epithelial invasion at the flap periphery. Fibrotic scar nasal to the flap periphery.

b Epithelial invasion cysts containing stillidentifiable epithelial cells. c Large epithelial invasion cysts containing dead epithelial cells surrounded by small cysts

Fig. 5.84 Refractive surgery: laser-assisted in situ keratomileusis (LASIK). Confocal microscopy image

of epithelial invasion at the interface with the stroma

b

c

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Chapter 5 Confocal Laser Scanning In Vivo Microscopy

 

 

Fig. 5.85 Refractive surgery: laser-assisted in situ keratomileusis (LASIK). Confocal microscopy image of keratocyte activation 1 week after LASIK

Fig. 5.86 Refractive surgery: laser-assisted in situ keratomileusis (LASIK). Confocal microscopy image of keratocyte activation 1 month after LASIK

a

b

Fig. 5.87 Refractive surgery: laser-assisted in situ keratomileusis. a, b Early after flap creation with femtosecond laser (IntraLase) showing the existence

of regularly disposed spots, probably corresponding to the microcysts created by laser impact. These spots disappear in the weeks following the procedure

5.5 Stroma

89

 

 

a

a

b

b

c

c

Fig. 5.88 Refractive surgery: laser-assisted in situ keratomileusis (LASIK). a Slit-lamp photograph of brightly reflective deposits at the LASIK interface. b, c Confocal in vivo microscopy image of these reflective deposits at the LASIK interface

Fig. 5.89 Refractive surgery: laser-assisted in situ keratomileusis (LASIK). a Slit-lamp photograph of the flap margin after the IntraLase procedure. b Corresponding confocal in vivo microscopy image. c Confocal in vivo microscopy image showing the flap margin created by the mechanical microkeratome. Note the less clearly recognizable incision compared with IntraLase

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Chapter 5 Confocal Laser Scanning In Vivo Microscopy

 

 

a

b

Fig. 5.90 Refractive surgery: laser-assisted in situ keratomileusis. a Slit-lamp photograph of an epithe-

lial cyst induced by an inclusion at the flap periphery. b Corresponding confocal in vivo microscopy image

a

b

Fig. 5.91 Refractive surgery: laser-assisted in situ keratomileusis at 6 months. Corneal nerves are again visible ahead of the interface

5.5 Stroma

91

 

 

Fig. 5.92 Refractive surgery: laser-assisted in situ keratomileusis. a Slit-lamp photograph of fibrosis at the flap margin. b Corresponding confocal

in vivo microscopy image

a

b

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Chapter 5 Confocal Laser Scanning In Vivo Microscopy

 

 

a

b

Fig. 5.93 Refractive surgery: complications of laser-assisted in situ keratomileusis.

a Slit-lamp photograph of fibrosis at the interface. c b, c Corresponding confocal in vivo microscopy

image