5 курс / Онкология / Оптимизация_хирургического_метода_лечения_миомы_матки

202

pro-apoptotic markers of aging increased in the area of post-surgery uterine scar

with increasing age of women.

Figure 3.6. – Comparative assessment of p16 protein expression pattern in the post-surgery scar area in women of different ages.

* – p 0.05 – compared to group 1

Figure 3.7 – Comparative assessment of mean p16 expression brightness of in the post-surgery scar area in women of different ages.

* – p 0.05 – compared to group 1

Рекомендовано к изучению сайтом МедУнивер - https://meduniver.com/

204

А B Figure 3.8 – VEGF expression in the two age groups

A – Group 1, B – Group 2. Cell nuclei were stained with DAPI – blue fluorescence, VEGF expression – red fluorescence (Alexa 647), ×200

А B Figure 3.9 – VEGFR expression in the two age groups.

А – Group 1, B – Group 2. Cell nuclei were stained with DAPI – blue fluorescence, VEGFR – red fluorescence (Alexa 647), ×200

According to the literature, it is known that neoangiogenesis factors are regulators of tissue repair, depending on the structure and receptor specificity, they can also contribute to the activation of the inflammatory process and scarring of tissue. The results of morphometric analysis of VEGF and VEGFR showed a significant decrease in the expression area of these molecules by 1.5 and 5.1 times, respectively, in the tissue

Рекомендовано к изучению сайтом МедУнивер - https://meduniver.com/

Figure 3.11 – Comparative assessment of mean VEGF expression brightness in the post-surgery scar area in women of different ages

* – p 0.05 – compared with group 1

Figure 3.12 – Comparative assessment of VEGFR expression pattern in the post-surgery scar area in women of different ages * – p 0.05 – compared to group 1

Рекомендовано к изучению сайтом МедУнивер - https://meduniver.com/

208

A.I. Shapovalova, V.O. Polyakova, T.S. Kleimenova // Doctor. – 2021. – Vol.32, N 9. –

P.76-79 [71].

3.5Type II collagen expression in the post-surgery uterine scar area

in women of different ages

It is known that collagen plays a significant role in cell proliferation, differentiation and migration, which can indirectly lead to thinning of the uterine wall. During the study, it was found that the expression area of type II collagen in the area of the post-surgery scar on the uterus in patients in the 1st age group was 7.81%, in the 2nd group – 8.53% and did not differ significantly (Figure 3.14).

Figure 3.14 – Comparative assessment of type II collagen expression pattern in the post-surgery scar area in women of different ages

This may be due to the fact that the synthesis of type II collagen in the uterine scar tissue does not change with age. According to the literature, there is a change in the localization of collagens of types I, III, IV, V, VI in the uterine tissue. At the same time, it was noted that the distribution and amount of type II collagen in the stroma of the organ has no age characteristics [75], which is consistent with the results we obtained.

Рекомендовано к изучению сайтом МедУнивер - https://meduniver.com/

209

The microphotographs show the results of an immunohistochemical study of the expression of type II collagen in the area of a post-surgery scar on the uterus after conservative myomectomy in the study groups (Figure 3.15).

А B

Figure 3.15 – Type II collagen expression in two age groups.

A – 1 group. B – 2 group. Cell nuclei were stained with DAPI – blue fluorescence, type II collagen expression – red fluorescence (Alexa 647), ×200

Morphometric analysis data revealed a significant 1.4-fold decrease in the average brightness of type II collagen expression in post-surgery uterine scar samples in women of group 2 compared with this indicator in group 1 (Figure 3.16).

Figure 3.16 – Comparative assessment of mean type II collagen expression brightness in the post-surgery scar area in women of different ages

* – p 0.05 – compared with group 1

210

The results obtained indicate that the rate of formation of the collagen component of the uterine tissue decreases with age, which complicates the healing time of the postsurgery scar. Thus, the results obtained allow us to form recommendations for pregnancy planning in older women after laparoscopic myomectomy, which is associated with the development of possible complications in the area of post-surgery scar on the uterus.

Specific results reported in Chapter 3 are published in the following papers:

Age features of the expression of signal molecules – protein p53, collagen type II, VEGF and VEGFR in bioptates of intact myometry in uterine myoma / A.I. Shapovalova, A.A. Tsypurdeeva, M.I. Kakhiani, E.N. Popov, V.O. Polyakova // Molekulyarnaya meditsina. – 2019. – Vol.17, N 9. – P. 60–63 [14].

Shapovalova, A.I. Age-related changes in expression levels of markers of close contacts in women after myomectomy / A.I. Shapovalova, V.O. Polyakova, T.S. Kleimenova // Siberian Journal of Life Sciences and Agriculture. – 2021. – Vol.13, N 2. – P. 32-46 [67].

Shapovalova, A.I. Expression of signaling molecules (p53, type II collagen, VEGF and VEGFR) in biopsies of intact myometrium in women of different ages / A.I. Shapovalova, V.O. Polyakova, T.S. Kleimenova // Doctor. – 2021. – Vol.32, N 9. –

P.76-79 [71].

3.6Proliferative PCNA protein expression of in the post-surgery uterine scar area

in women of different ages

The study of the synthesis of the PCNA molecule in the uterine scar tissue allows

of PCNA expression in the area of a post-surgery scar on the uterus after conservative myomectomy in women of age groups 1 and 2 (Figure 3.17).

Рекомендовано к изучению сайтом МедУнивер - https://meduniver.com/