- •General microbiology (continued from spring semester)
- •The Scheme of Complement Fixation Test
- •The Scheme of Flocculation Test
- •Special microbiology section 1: special bacteriology (Enteric bacterial pathogens)
- •The Protocol of the Blood Culture Bacteriological Examination
- •In Suspected Enteric Fever
- •The Protocol of the Bacteriological Examination of Vomit Specimens
- •In Suspected Salmonellosis
- •The Scheme of Bacteriological Examination of Stool Specimens
- •In Enteric Escherichiosis (infant’s diarrhea)
- •The Scheme of Bacteriological Examination of Stool Specimens in Shigellosis
- •The Kaufmann-White’s Scheme of Salmonellae Classification
- •Section 2: special bacteriology
- •The Most Common Pathogens of Streptococcal Human Diseases
- •The Scheme of iha for Botulinum Toxin Identification
- •Determination of Average Concentration of Bacteria in Urine Sample
- •* Zoonoses
- •The Scheme of the Huddleson’s Test
- •The Scheme of the Wright’s Test
- •The Scheme of the Ascoli’s Test (the Precipitation Test for Diagnosis of Anthrax)
- •* Special virology
- •Identification of viruses. Serological tests used for making diagnosis of viral diseases. Viruses - the causative agents of respiratory tract diseases. Rabies virus. Smallpox virus
- •The Scheme of vha-test for Virus Indication in the Allantoic Fluid
- •The Results of the hai Test for the Serological Examination
- •Cpe of polioviruses (destruction of monolayer)
- •The Scheme of elisa for hBs antigen Detection in a Patient’s Serum
Determination of Average Concentration of Bacteria in Urine Sample
Number of colonies in sectors on MPA |
The average concentration of bacteria per 1ml |
|||
Sector A |
Sector 1 |
Sector 2 |
Sector 3 |
|
1-6 |
-- |
-- |
-- |
<1,000 |
8-20 |
-- |
-- |
-- |
3,000 |
20-30 |
-- |
-- |
-- |
5,000 |
30-60 |
-- |
-- |
-- |
10,000 |
70-80 |
-- |
-- |
-- |
50,000 |
100-150 |
6-10 |
-- |
-- |
100,000 |
>200 |
20-30 |
-- |
-- |
500,000 |
>200 |
40-60 |
-- |
-- |
1,000 000 (106) |
>200 |
100-140 |
10-20 |
-- |
5,000 000 (5x106) |
>200 |
>200 |
30-40 |
-- |
10,000 000 (107) |
>200 |
>200 |
60-80 |
1-8 |
over 100,000 000 (108) |
* Zoonoses
LESSON 10 Date:
BACTERIOLOGICAL EXAMINATION of CLINICAL SPECIMENS (continued from lesson 9).
BACTERIA – the CAUSATIVE AGENTS OF ZOONOTIC DISEASES (plague, tularemia, brucellosis, and anthrax)
Table 21
The Scheme of Tube Agglutination Test for Serological Diagnosis of Tularemia
Ingredients |
1 |
2 |
3 |
4 |
5 |
serum control |
antigen control |
Isotonic solution (ml) |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
-- |
1.0 |
Patient’s serum 1: 25 (ml) |
1.0 |
|
|
|
pour out |
1.0 |
-- |
The serum dilutions |
1:50 |
1:100 |
1:200 |
1:400 |
1:800 |
1:25 |
-- |
Diagnosticum (drops) |
3 |
3 |
3 |
3 |
3 |
-- |
3 |
Results: |
|
|
|
|
|
|
|
CONCLUSION: The agglutination test is ______________ (positive/negative). The titer of antibodies against antigens of Francicella tularensis is______. The diagnosis of tularemia is (is not) confirmed. |
|||||||
Table 22
The Scheme of the Huddleson’s Test
(the Slide Agglutination Test for Serological Diagnosis of Brucellosis)
Serum - 0.08 ml Antigen - 0.03 ml Obtained dilution 1:50 |
Serum - 0.04 ml Antigen - 0.03 ml Obtained dilution 1:100 |
Serum - 0.02 ml Antigen - 0.03 ml Obtained dilution 1:200 |
Result: |
Result: |
Result: |
Serum - 0.01 ml Antigen - 0.03 ml Obtained dilution 1:400 |
Serum - 0.03 ml Isotonic saline - 0.03 ml Serum control |
Antigen - 0.03 ml Isotonic saline – 0.03 ml Antigen control |
Result: |
Result: |
Result: |
CONCLUSION: The slide agglutination test is _____________ (positive/negative). The titer of antibodies against antigens of Brucella is__________.
|
||
Table 23