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The Scheme of Flocculation Test

Tube #

1

2

3

4

5

Diphtheria Toxoid (ml)

2.0

2.0

2.0

2.0

2.0

Diphtheria antiserum (ml) (400 AU/ml)

0.16

0.18

0.2

0.22

0.24

Water bath 40-45˚C for 45 min

Results (flocculation)

CONCLUSION: The Diphtheria Toxoid Titer is __________ Lf/ml

Picture 3. Classification of vaccines

Picture 4. Classification of medical immunobiological preparations used for passive immunization

Special microbiology section 1: special bacteriology (Enteric bacterial pathogens)

LESSON 4 Date:

The SALMONELLAE – the causative agents of typhoid fever and salmonellosis

Table 6

The Protocol of the Blood Culture Bacteriological Examination

In Suspected Enteric Fever

Examination day (step)

Manipulations

Results and conclusions

The First

Inoculation of the 10% bile broth with the patient's blood (5 ml of blood tested is added to 50 ml of the broth, 1:10 ratio).

The Second

The Third

The Fourth

1.

2.

3.

4.

CONCLUSION: the isolated microbe is identified as ___________________ that confirms the diagnosis of __________________. Phagotype: ______.

Table 7

The Protocol of the Bacteriological Examination of Vomit Specimens

In Suspected Salmonellosis

Examination day (step)

Manipulations

Results and conclusions

The First

The Second

The Third

1.

2.

3.

CONCLUSION: the isolated microbe is identified as __________________________ that confirms the diagnosis of __________________.

LESSON 5 Date:

The SALMONELLAE (continued from lesson 4). The ESCHERICHIAE (causative agents of escherichiosis). The SHIGELLAE (causative agents of bacterial dysentery)

Table 8

The Scheme of Bacteriological Examination of Stool Specimens

In Enteric Escherichiosis (infant’s diarrhea)

Examination day (step)

Manipulations

Results and conclusions

The First

The Second

The Third

CONCLUSION: the isolated microbe is identified as __________________________ serogroup _______ that confirms the diagnosis of _________________.

Table 9

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