- •General microbiology (continued from spring semester)
- •The Scheme of Complement Fixation Test
- •The Scheme of Flocculation Test
- •Special microbiology section 1: special bacteriology (Enteric bacterial pathogens)
- •The Protocol of the Blood Culture Bacteriological Examination
- •In Suspected Enteric Fever
- •The Protocol of the Bacteriological Examination of Vomit Specimens
- •In Suspected Salmonellosis
- •The Scheme of Bacteriological Examination of Stool Specimens
- •In Enteric Escherichiosis (infant’s diarrhea)
- •The Scheme of Bacteriological Examination of Stool Specimens in Shigellosis
- •The Kaufmann-White’s Scheme of Salmonellae Classification
- •Section 2: special bacteriology
- •The Most Common Pathogens of Streptococcal Human Diseases
- •The Scheme of iha for Botulinum Toxin Identification
- •Determination of Average Concentration of Bacteria in Urine Sample
- •* Zoonoses
- •The Scheme of the Huddleson’s Test
- •The Scheme of the Wright’s Test
- •The Scheme of the Ascoli’s Test (the Precipitation Test for Diagnosis of Anthrax)
- •* Special virology
- •Identification of viruses. Serological tests used for making diagnosis of viral diseases. Viruses - the causative agents of respiratory tract diseases. Rabies virus. Smallpox virus
- •The Scheme of vha-test for Virus Indication in the Allantoic Fluid
- •The Results of the hai Test for the Serological Examination
- •Cpe of polioviruses (destruction of monolayer)
- •The Scheme of elisa for hBs antigen Detection in a Patient’s Serum
The Scheme of Flocculation Test
Tube # |
1 |
2 |
3 |
4 |
5 |
Diphtheria Toxoid (ml) |
2.0 |
2.0 |
2.0 |
2.0 |
2.0 |
Diphtheria antiserum (ml) (400 AU/ml) |
0.16 |
0.18 |
0.2 |
0.22 |
0.24 |
Water bath 40-45˚C for 45 min |
|||||
Results (flocculation) |
|
|
|
|
|
CONCLUSION: The Diphtheria Toxoid Titer is __________ Lf/ml
|
|||||
Picture
3. Classification
of vaccines
Picture
4. Classification
of
medical
immunobiological preparations used for passive immunization
Special microbiology section 1: special bacteriology (Enteric bacterial pathogens)
LESSON 4 Date:
The SALMONELLAE – the causative agents of typhoid fever and salmonellosis
Table 6
The Protocol of the Blood Culture Bacteriological Examination
In Suspected Enteric Fever
Examination day (step) |
Manipulations |
Results and conclusions |
The First |
Inoculation of the 10% bile broth with the patient's blood (5 ml of blood tested is added to 50 ml of the broth, 1:10 ratio). |
|
The Second |
|
|
The Third |
|
|
The Fourth |
1.
2.
3.
4.
|
|
CONCLUSION: the isolated microbe is identified as ___________________ that confirms the diagnosis of __________________. Phagotype: ______.
|
||
Table 7
The Protocol of the Bacteriological Examination of Vomit Specimens
In Suspected Salmonellosis
Examination day (step) |
Manipulations |
Results and conclusions |
The First |
|
|
The Second |
|
|
The Third |
1.
2.
3.
|
|
CONCLUSION: the isolated microbe is identified as __________________________ that confirms the diagnosis of __________________.
|
||
LESSON 5 Date:
The SALMONELLAE (continued from lesson 4). The ESCHERICHIAE (causative agents of escherichiosis). The SHIGELLAE (causative agents of bacterial dysentery)
Table 8
The Scheme of Bacteriological Examination of Stool Specimens
In Enteric Escherichiosis (infant’s diarrhea)
Examination day (step) |
Manipulations |
Results and conclusions |
The First |
|
|
The Second |
|
|
The Third |
|
|
CONCLUSION: the isolated microbe is identified as __________________________ serogroup _______ that confirms the diagnosis of _________________.
|
||
Table 9
