- •Introduction
- •Precautions for laboratory works
- •Chapter 1
- •Modern methods for raw materials analysis at the biotechnological industries
- •Laboratory work 1
- •Detemination of plant originated raw material acidity. Plant extracts and juices acidity definition
- •Main tasks
- •Basic theoretical information
- •1.1. Determination of plant extracts and juices acidity with the help of pH-meter
- •1.2. Fast determination of active acidity of fruit juice by the test paper
- •1.3. Determination of titrated acidity of plant raw materials and extracts
- •Analysis of obtained results
- •Starch, yeast and flour acidity determination.
- •Main tasks
- •Basic theoretical information
- •Normal values of acidity for different sorts of starch
- •2.1. Determination of starch acidity
- •2.2. Determination of yeast acidity
- •2.3. Determination of the flour acidity
- •Enzymes activity determination Determining of malt of α-amylaze enzymatic activity Malt extractives definition
- •3.1. The determination of amylolytic enzyme activity of fresh malt by visual colorimetric method
- •3.2. Determination of extract content of malt
- •Experimental data processing
- •3.3. Determination of malt sugarising duration
- •Quantification of water-ethanol solutions, areometric method. Definition of ethanol aqueous solution strength
- •Main tasks
- •Basic theoretical information
- •4.1. Determination of aqueous ethanol solutions concentration with the help of glass alcoholmeter
- •Work procedure
- •Ethanol content at the different temperatures and alcoholmeter indications
- •4.2. Waterless ethanol calculation
- •Experimental data processing
- •Chapter 2 Modern methods for analysis of finished and waste products at the biotechnological plants
- •Chemical methods for carbohydrates determination. Determination of reducing sugars by Max-Müller
- •Water extracts preparing for carbohydrates quantification. Determination of starch content in potato by Evers
- •Main tasks
- •Basic theoretical information
- •6.1. Determination of starch content in potatoes by Evers
- •Polarimetric method for carbohydrates analysis Starch content determination in cereals by Archipovich
- •8.1. Defining the properties of vitamins b2, b3, b6,
- •8.2. The color reaction of vitamin b6 chloride of iron (III)
- •8.3. The color reaction of vitamin b3 with copper acetate
- •8.4. Determination of carotene by photometric method
- •8.5. Determination of vitamin c
- •List of literature
- •Contents
- •Introduction 3
- •Методи аналізу біотехнологічних виробництв
- •6.051401 «Біотехнологія»
Introduction
The development of biotechnology allows significant intensification of manufacturing, improve the efficiency of natural resources, solve environmental problems and create new sources of energy. The possibilities of biotechnology in case of international cooperation experts can address the world's critical problems caused by deficiency of protein and energy, prevention of dangerous diseases, environmental protection.
Due to analytical methods of biotechnological industries society has the opportunity to consciously control its economic and technology policy, seeking release of products of high quality. With increasing the competition should increase product quality, but it requires strict control at all stages of obtaining raw materials, production, storage and sales.
The main purpose of laboratory guidance is mastering the basic methods of analysis in biotechnology industries, sequence of raw materials and finished products control, the study of factors and conditions affecting the quality of products and methods of assessment. Themes of laboratory works are chosen to prepare the expert to work on modern biotechnology industries to future specialist able to use the methods of determining quality indicators and assess its compliance with specified requirements; identify indicators of quality of raw materials and finished products according to the requirements of existing standards; identify factors that influence the measurement results.
Laboratory classes of training course “Analytical Methods for Biotechnological Industry” are carried out according to the curriculum for bachelors full-time and distance learning direction 6.051401 "Biotechnology" specialties 7 / 8.05140103 "Pharmaceutical Biotechnology" and 7 / 8.05140105 "Environmental Biotechnology and Bioenergy".
Precautions for laboratory works
In biochemical laboratory it is necessary to follow the rules of behavior and work to ensure the safety of people. Therefore on the first lesson, student must read the rules of safety and sign the register.
At the classes in the laboratory the duty is assigned, who is responsible for the maintenance of order and cleanliness. On the desktops there should be no unnecessary objects, bags, briefcases. Reagents occasionally scattered on the table and solvents spills should be immediately removed. It is strictly forbidden to eat in the laboratory. Do not use substances present in glasses without labels.
Laboratory work can begin only after careful studying of techniques and learn how to work on the appropriate manner. You can work only with proper equipment and appliances.
Careless handling of acids and alkalis can lead to accidents, damage to the eyes, skin face and respiratory tract. For eye protection when working with concentrated acids and alkalis should wear safety glasses. When diluting concentrated acids must follow the order of mixing, slowly pour acid into water.
Experiments with caustic, toxic or highly aromatic substances permitted to hold only in a laminar box.
Special care requires working with arsenic, mercury and other poisons. Residues containing toxic substances cannot be thrown in a bowl or basket. They are collected in special glass. After the experiments it is necessary to wash your hands thoroughly.
Glassware and instruments (including thermometers) should be handled with care, do not put on the edge of the table, do not touch with elbows. Pieces of broken glassware should be removed immediately. After the classes it is necessary to clean workplace close all gas and faucets, turn off electrical appliances.
Duty student report to laboratory technician (engineer), the laboratory is in the proper order.
