Медицинская вирусология / Букринская А.Г., Жданов В.М. Молекулярные основы патогенности вирусов
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ero 61,wa 06Hapy)1<eHa H ,ll,mr F-6CJ1Ka n11pyca Cen,ll,afl [Tashi ro M. et al., 1988].
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B 1iaTorcHe3e rpm111mHoil. 1111cj)eKnm1 na*IIYIQ po.m, Mory1 HrpaTb cep11H0BblC np0TC33hl, ceKpen1pyeMhlC HCKOTOpblt,Hl 6aKTCPilflMH, HarrpHMCJ) 30,710THCTbIM CTaqJIIJIOKOKKOM, KOTOj)bIH <IaCTO BLl,ll,CJJHIOT OT 60JibilblX C rpHIIII03HOII ITIICBMOHHeii. Ha JIH'IHC 6aKTepnaJJLHoro rt)epMe11Ta, ocylll,eCTBJJHIOlll.ero npoTeo JJHTHIJecKy10 aKTHB3U:IllO rrnpyca, cnoco6CTBYCT MIIO)KCCTBeH HblM UHKJiaM penJIIJK3UIIII BHpyca B opraHH3MC ;-1.a2Ke npn OTCYTCTBHH crreumjrn11ecKOii llj)()TCa3bl. 3apa,Ke1me MbllllClf 0,1], H0BpCMCIIHO C !3HpycoM rp1rnrra 30JIOTHCTblM CTacjrnJJOKOKKOM npHBO,QIIJ!O K j)C3KOMY YBCJllfllCllll!0 THTpa !3Hpyca B JierKIIX II rn6em1 MblillC!f OT rpHilll03HOH HHeBMOHHH. l1Hrn61nop1,1 npo
TC33, Tairne K3K c:-aMJIHOKanpo110BaH KHCJIOTa H anpOTllHHHbl [)K11pHoB 0. II. I! AP-, 1984], JwynenTIJH H ,n:p. [Tashiro M.
et aI., 1987], rrpHB0JUIJrn K 6.110Frnp0B,rnmo nape311H11H HA KJ1e T0'l!lhlM11 n GaKTcp11a.nhHbJM11 np<nea3aM!I LTashiro M. ct al.,
117
1987] H npe,Q0TBpamaJIH rn6eJib Mbllllei'i. OJI.HOBpeMeHIIOe 3a pa1KCHHC Mbm1e11 BupycoM rpHmrn I I 6aKTepuerr, npo,11.ynnpy10meii 3K30npoTea3y, Serratia rnarcescens, npIIB0JJ.H.r10 K pe3K0· MY ycHJICHJ:l!0 .neTa.!JbI!0r0 }l,eii:cTBIIH BHpyca, B 0CH0Be K0T0poro
.TielliaJia aKTHB311IIH nJia3MI!HoreHa Jl.0 IlJ133MHH3 6aKTepHaJlb HOH npoTea3ott; nJia3MHH Hape3aJl BHpycm,n'i HA H cnoco6CT· B0BaJI rcttepamrnaIJ,HH HHCpeK11,m1 [Akaikc T. et al., 1989]. B3aIIMOJl.CHCTBHe MC,!{Jl.Y BHpycoM rpmma H 6aKTCpHHMH II tta6JlIOJI.aCMb!H CHIIeprnqecKHH 3qHpCKT 061,HCHHIOT qaCT0C OC.f!Oli<HCHHe rprmrra 6aKTCpHaJibHbIMH rrnq>CKl(HHMH H YBCJ!H qem1e JICT3JlbH0CTH cpe,ru1 60.ribHbIX xpo!Ul'ICCKHMH pecmrpa T0pHblMH HIHj>eKLUI5!MH BO BpeMH rpHTITI03HblX 3TIH,1l,CMHH.
napaMHKCOBHpycbl. y napaMHKC0BHpycon npoTe0.rIHT!IlJC CKaH 8KTHB81[IHI CBH33Ha C Hape33HHCM npe,runeCTBCHHHKa 6e.riKa CJlHHHHH F0 Ha q.iynK11110HaJibHhIH 6eJioK F1 H 6eJIOK F2, KOTOpbll'i, 110-BH,l[I!M0MY, He HrpaeT p0JIJI B rrp0HHKH0Bemrn BH pyca B K.'lCTKy. Hape3aHHe ocyni:ecTB.r!HCTC5!, KaK H B c.riyqae m1pyca rp1mna, K.11eT0tIHblMH TpHITCHHOTJO,l[06HbIMH cpepMCHTa MH. 3TH cpepMCHTbI B }l,0cTaTO'IHOM KO.!l!-Jl!CCTBe06yc.rioB,TIIIBaIOT TPOilII3M BHpycoB K orrpe,11.elICHHblM TK3HHM If opraHaM, a T3K· )Ke CTCITeHh BHpy.rieHTl!OCTII, CB5133HHYIO C re11epaJIH3aUHeH BII pyca. HanpIIMep, B ocHoBc TpomI3Ma BHpyca Ce1u1af1 K J1e ro1rnott TKaHH MblllleH .r!C)KI!T BbIC0I<aH K0IIIl,CHTpal(llH B TKaHH TpHrrcHHOITOJL06HbIX qJepMeHTOB [Tashiro M., Homma M., 1983]. flpu 33MCHC 3Ml!II0KHCJ10THblX 0CT3TK0B 13 y1IaC'I'KC Hape3aHTIH F-6e.riKa B rrponecce Hacc11poBaHHH Bnpyca B npII· cyTCTBHH XllM0TpHITCIIHa 6hIJIII ITOJ1Y1ICHbl MyTaHThI, K0TOpble aKTJIBHpOBaJlHCb He TPIIl1Cl1H0M, a XHMl!OTpimCHHOM. Y T8KIIX MyTa!ITOB 061-iapy)JU!BaJIIICb l.J.BC 33MeHhl B C0C)LHHHTCJlbH0M IlCITTlIAC [Hsu M. et al., 1987]. MyTaHTbI 6bI.T!Il cnoco6Hbl T0.rlbKO K 0)].1-IOMY ll,IlKJiy penp0JJ.YKl( liH B .!lCrKIIX MblIIIeH, r,r e He 6bL10 q.iepMCHTOB JJJ!Sl ero aKT1maurm. I1mjlCKI[l1H He npHB0· AII.ria K nopa)l{€'HHIO ,'lerKIIX. 3aMeHa O)LHOfI aMIIH0KHC.rIOTbl n
yqacTKC nape3amrn F-6eJIKa mipyca Ce11Aaii npusomrna K no HBJ1e1-11110 ycT011,mBocTH K Tp1rncIIHY [ Itoh M. et al., 1987].
MyTaHTbI nnpyca CeH,l!at1 n NDV rro F-6eJrny 06.riana.riH pa3Hof1 cTeneHbI0 IIHTonaTIITICCK0ro 3cl>q.ieKTa u pa3.rIHlJaJmcb no BIIpyJICHTH0CTII B 33BIICIIM0CTH OT cmrnaionieH aKTHBHOCTII F-6eJIKa, KOT0paH B CBOIO oqepe)J.h onpe,1l,CJI5IJJaCb npoTC0JIII·
TIJllCCKHM Hape33IIHCM 6e.11Ka Fo Kl!CTO'!Hb!Mll npoTeasaMH [Toyoda T. et al., 1987; Tashiro M. et al., 1988]. Cxo,11.nb1e
JWHHhie 6bIJlH noJiyqerrn n ,rvrn mipyca rraponna: F-6eJJOK mipyJieIITHOI'0 1UT[lMM8 0TJHlll3JlC5! OT F-6eJIK3 HCB1IpyJ1eHTIIOro
ruTaMMa 14 aMHH0KHCJIOTHhIMII ocTaTirnMH [Elango N. et al.,
1989].
118
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120