Схема строения α β TCR рецептора
α цепь β цепь
Вариабельный район (V)
Константный район (С)
Трансмембранный
район
Цитоплазматический
Дисульфидный хвост мостик
Реаранжировка α и β цепей TCR
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Vβx70-80 |
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Реаранжировка |
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генов |
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Синтез мРНК |
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V J |
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C |
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Vβx52 |
Dβ1 Jβ1x6 |
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Cβ0 Cβ1 Dβ2 |
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Jβ2x7 |
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Cβ2 |
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Реаранжировка |
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генов |
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Синтез мРНК |
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Альтернативный |
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сплайсинг мРНК |
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V D1 J1 C0 |
C1 |
V D1 J1 C1 |
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Векторы pTα и pTβ, использованные для клонирования α и β цепей TCR
pTα |
pTβ |
Вектор pTZ57R, использованный для клонирования α и β цепей TCR
Определение геномной ДНК α и β цепей TCR в векторе pTZ57R
1D1 β genome in pTZ57R vector |
1D1 α genome in pTZ57R vector |
+ и – обозначены позитивные и негативные по содержанию вставки клоны. М – маркер молекулярного веса
Клонирование α цепи TCR в pTα вектор
Sma I |
Sac II |
α цепь
+
Sma I |
Sac II |
α цепь
pTα |
pTα |
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Нуклеотидная последовательность геномной ДНК β цепи TCR
1D1 β genome
atgaacaagtgggttttctggctgggtaaccctttgtctccttactgtaggtaaggctctgggctctctgggttcctgttgccgagt gcacatcttaacccccctgccctgggtagcagtgccaaaccccttccagactgattctttttcttttccagagaccacacatggt gatggtggcatcattactcagacacccaaattcctgattggtcaggaagggcaaaaactgaccttgaaatgtcaacagaatttc aatcatgatacaatgtactggtaccgacaggattcagggaaaggattgagactgatctactattcaataactgaaaacgatcttc aaaaaggcgatctatctgaaggctatgatgcgtctcgagagaagaagtcatctttttctctcactgtgacatctgcccagaaga acgagatggccgtttttctctgtgccagcaccccgacagaactctcctatgaacagtacttcggtcccggcaccaggctcacg gttttaggtaagattcacatctctcgcttccacccaaattcctgggtccctggagtagtttggagtagaccagggttagggaatct ccgggagggaaatcgatagtaggagacagctctcaacttcaccctttctgggggagcgggatgaaaagggattgggtgcca aacgttgttcgctctctcgcaagccaggacaaggtaggtttctttctcttttctctgaggtatgcatgcactgggtgggtggtgaa gaaaaagaattaattaggaccgcggaagcaaacttggttttgaaaataaatagccggcacaaacccgcgggaagaatctag atgcattcgncgagacacggctagc
V район обазначен зеленым
D район обозначен фиолетовым
J район обозначен голубым
N нуклеотиды обозначены коричневым цветом.
Нуклеотидная последовательность геномной ДНК α цепи TCR
1D1 α genome
ctagccggtcacgncgaatgcatctagattccagcccggggnacaggngccatgcagaggaacctgggagctgtgctgggg attctgtgggtgcagatttgctgtgagttgtgctaagttcctattagagattctccagaaatgtcggtggtggagggggagaagttg atggaaatctcctgtgcactcttcctcatgggtgtctcctggggtagttgactggtgtgagtgaaaatctctctttgattttggtttgctt ttctgttttcaagcaggggtgagcggagataaggtgaaacaaagtccctcagcgctgagtctccaagaaggaaccaattctgctc tgagatgcaatttttctatcgccgcgacaactgtgcagtggttcctacagaatcccaggggcagcctcatcaatcttttttacctggt tccaggaacaaaggagaatgggaggttaaagtcagcattcgattctaaggagagctacagcaccctgcacatcagggatgccc agctggaggactcaggcacttacttctgtgctgctgatctcatgtctaattacaacgtgctttacttcggatctggcaccaaactcac tgtagagccaagtaagttattgttacccacccattatgctaaattcctctactcgctctatatttgctttgagttctctttacttggatcctt gtccaatgttgtatca
V район обазначен зеленым
J район обозначен голубым
N нуклеотиды обозначены коричневым цветом.